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1. WO1987006261 - RECOMBINANT - RNA PACKAGING SYSTEM

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Publication Number WO/1987/006261
Publication Date 22.10.1987
International Application No. PCT/GB1987/000249
International Filing Date 13.04.1987
IPC
C12N 15/82 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
C12N 7/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
CPC
C12N 15/8206
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8206by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
C12N 2770/00023
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2770ssRNA Viruses positive-sense
00011ssRNA Viruses positive-sense
00023Virus like particles [VLP]
C12N 2770/00043
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2770ssRNA Viruses positive-sense
00011ssRNA Viruses positive-sense
00041Use of virus, viral particle or viral elements as a vector
00043viral genome or elements thereof as genetic vector
C12N 7/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
Applicants
  • DIATECH LIMITED [GB]/[GB] (AllExceptUS)
  • WILSON, Thomas, Michael, Aubrey [GB]/[GB] (UsOnly)
Inventors
  • WILSON, Thomas, Michael, Aubrey
Agents
  • ELKINGTON AND FIFE
Priority Data
860885011.04.1986GB
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) RECOMBINANT - RNA PACKAGING SYSTEM
(FR) SYSTEME DE CONDITIONNEMENT D'ARN RECOMBINANT
Abstract
(EN) A chimaeric RNA comprises the origin of assembly sequence of a helical rod-shaped virus such as tobacco mosaic virus together with at least one sequence coding for a foreign protein. The chimaeric RNA can be produced by producing cloned cDNA copies of the RNA origin of assembly sequence and cloned DNA sequences coding for a foreign protein, ligating the cloned DNA sequences in the correct orientation and transcribing the recombinant DNA in a suitable transcription vector system. When the chimaeric RNA is assembled in a preparation of the coat protein of the virus whose origin of assembly sequence is included in the chimaeric RNA, a pseudovirus particle is produced in which the chimaeric RNA is encapsidated by the virus coat protein. The pseudovirus particle can be used as a general means for protecting the RNA or as a vector for expression of the RNA in a wide variety of hosts.
(FR) Un ARN chimérique comprend l'origine de la séquence d'assemblage d'un virus en forme de tige hélicoïdale tel que le virus de la mosaïque du tabac avec au moins une séquence codant une protéine étrangère. L'ARN chimérique peut être obtenu en produisant des copies d'ADNc clonées de l'origine ARN de la séquence d'assemblage et des séquences d'ADN clonées codant une protéine étrangère, par ligation des séquences d'ADN clonées dans l'orientation correcte et transcription de l'ADN recombinant dans un système vectoriel de transcription appropriée. Lorsque l'ARN chimérique est assemblé dans une préparation de la protéine d'enrobage du virus dont l'origine de la séquence d'assemblage est incorporée dans l'ARN chimérique, une particule de pseudovirus est produite dans laquelle l'ARN chimérique est encapsulée par la protéine d'enrobage du virus. La particule de pseudovirus peut être utilisée comme moyen général pour protéger l'ARN ou comme vecteur d'expression de l'ARN dans une grande variété d'hôtes.
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