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1. (WO1987003690) PARTICLE-BOUND BINDING COMPONENT IMMUNOASSAY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1987/003690 International Application No.: PCT/US1986/002637
Publication Date: 18.06.1987 International Filing Date: 10.12.1986
IPC:
G01N 33/538 (2006.01) ,G01N 33/543 (2006.01) ,G01N 33/558 (2006.01) ,G01N 33/569 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
536
with immune complex formed in liquid phase
537
with separation of immune complex from unbound antigen or antibody
538
by sorbent column, particles or resin strip
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
558
using diffusion or migration of antigen or antibody
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
569
for micro-organisms, e.g. protozoa, bacteria, viruses
Applicants:
MUREX CORPORATION [US/US]; Post Office Box 2003 Norcross, GA 30091, US
Inventors:
MARSHALL, David, Lee; US
Agent:
BERNSTEIN, Jason, A.; Post Office Box 2003 Norcross, GA 30091, US
Priority Data:
807,15710.12.1985US
Title (EN) PARTICLE-BOUND BINDING COMPONENT IMMUNOASSAY
(FR) IMMUNOANALYSE DE COMPOSANTS DE LIAISON LIES A DES PARTICULES
Abstract:
(EN) An immunoassay method for the detection or quantitation of an analyte suspected of being in a solution comprising: (a) combining said specimen, a first binding component, insoluble particles, and second binding component labelled with a signal generating material in a solid phase retention and separation apparatus having a sufficient pore size such that said particles are trapped within said filter yet permitting rapid passage of fluid therethrough in such a manner that an immunological reaction occurs if analyte is present in said specimen, resulting in the formation of an immunocomplex of insolubilized first binding component: analyte: second labelled binding component on or within said filter means; (b) separating bound from unbound material; and (c) determining the presence and/or amount of signal produced which is correlative with the amount of analyte present in the solution.
(FR) Procédé d'immunoanalyse pour la détection ou détermination quantitative d'un analyte que l'on soupçonne d'être présent dans une solution, ladite méthode consistant à: a) combiner ledit spécimen, un premier composant de liaison, des particules insolubles et un second composant de liaison marqué avec un matériau générateur de signaux dans un appareil de rétention et de séparation de phase solide ayant une dimension des pores suffisante pour que lesdites particules soient piégées dans le filtre tout en permettant un passage rapide du fluide au travers de ce filtre et qu'il s'ensuive une réaction immunologique si l'analyte est présent dans ledit spécimen, ce qui se traduit par la formation d'un immunocomplexe constitué du premier composant de liaison insolubilisé, de l'analyte, et du second composant de liaison marqué sur ou dans lesdits moyens de filtration; b) séparer le matériau lié du matériau non lié; et c) déterminer la présence et/ou la quantité de signal produit qui est en corrélation avec la quantité d'analytes présents dans la solution.
Designated States: AU, BR, FI, JP
European Patent Office (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0248892AU1987068395