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1. (WO1987003618) PROCESS FOR MANUFACTURE OF L-ASPARAGINASE FROM ERWINIA CAROTOVORA
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1987/003618 International Application No.: PCT/US1986/002665
Publication Date: 18.06.1987 International Filing Date: 11.12.1986
IPC:
C12N 9/82 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
78
acting on carbon to nitrogen bonds other than peptide bonds (3.5)
80
acting on amide bonds in linear amides
82
Asparaginase
Applicants:
UNITED STATES OF AMERICA, represented by THE UNITE [US/US]; D STATES DEPARTMENT OF COMMERCE; Washington, DC 20230, US
Inventors:
LEE, Shwu-Maan; US
ROSS, John, T.; US
WROBLE, Marie; US
Agent:
OLIFF, James, A.; Parkhurst & Oliff 277 S. Washington Street Alexandria, VA 22314, US
Priority Data:
808,72913.12.1985US
Title (EN) PROCESS FOR MANUFACTURE OF L-ASPARAGINASE FROM ERWINIA CAROTOVORA
(FR) PROCEDE DE PRODUCTION DE L-ASPARAGINASE A PARTIR DE L'ERWINIA CAROTOVORA
Abstract:
(EN) A process for the recovery and purification of L-asparaginase from $i(Erwinia carotovora) involves preparation of cellular acetone powder extract followed by either an ion exchange and affinity chromatography purification or by affinity chromatography alone. The column eluent is then dialyzed to produce substantially pure L-asparaginase.
(FR) Procédé d'extraction et de purification de L-asparaginase à partir de l'$i(Erwinia carotovora), comprenant la préparation d'un extrait de poudre d'acétone cellulaire suivie d'une étape de purification soit par chromatographie à échange d'ions et par affinité, soit par chromatographie par affinité uniquement. L'éluent dans la colonne est ensuite dialisé pour produire une L-asparaginase essentiellement pure.
Designated States: AU, JP
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU1987067734