Processing

Please wait...

Settings

Settings

Goto Application

1. WO1986001229 - METHOD OF REMOVING N-TERMINAL AMINO ACID RESIDUES FROM EUCARYOTIC POLYPETIDE ANALOGS AND POLYPEPTIDES PRODUCED THEREBY

Publication Number WO/1986/001229
Publication Date 27.02.1986
International Application No. PCT/US1985/001531
International Filing Date 09.08.1985
IPC
C07K 14/555 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
52Cytokines; Lymphokines; Interferons
555Interferons (IFN)
C07K 14/61 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
575Hormones
61Growth hormone (GH) (Somatotropin)
C12N 9/48 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
48acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
C12P 21/06 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
06produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
CPC
C07K 14/555
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
52Cytokines; Lymphokines; Interferons
555Interferons [IFN]
C07K 14/61
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
575Hormones
61Growth hormones [GH] (Somatotropin)
C12N 9/48
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
48acting on peptide bonds (3.4)
C12P 21/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
06produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Applicants
  • BIO-TECHNOLOGY GENERAL CORP. [US]/[US]
Inventors
  • BLUMBERG, Shmaryahu
  • BEN MEIR, Daniela
Agents
  • WHITE, John, P.
Priority Data
641,48816.08.1984US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF REMOVING N-TERMINAL AMINO ACID RESIDUES FROM EUCARYOTIC POLYPETIDE ANALOGS AND POLYPEPTIDES PRODUCED THEREBY
(FR) PROCEDE D'ELIMINATION DES ACIDES AMINES RESIDUELS A TERMINAL N D'ANALOGUES POLYPEPTIDES EUCARYOTES ET POLYPEPTIDES OBTENUS PAR CE PROCEDE
Abstract
(EN) A method of sequentially removing one or more N-terminal amino acid residues from an analog of a eucaryotic polypeptide synthesized in a foreign host, comprises contacting the eucaryotic polypeptide analog with aminopeptidase under suitable conditions permitting sequential removal of N-terminal amino acid residues, where the polypeptide analog contains amino acid residue or sequence of residues which blocks the action of an aminopeptidase located at a position other than the N-terminal end of the polypeptide analog. A specific embodiment of the invention concerns removing both an N-terminal methionine residue and its adjacent Leucine residue from a growth hormone analog produced in bacteria by contacting the growth hormone analog with $i(Aeromonas) aminopeptidase under suitable conditions permitting removal of the N-terminal methionine and Leucine residues. The invention also concerns eucaryotic polypeptide analogs produced in accordance with the methods of the invention.
(FR) Un procédé d'élimination séquentielle d'un ou plusieurs acides aminés résiduels d'un analogue d'un polypeptide eucaryote synthétisé dans un hôte étranger comprend la mise en contact de l'analogue polypeptide eucaryote avec de l'aminopeptide dans des conditions appropriées permettant l'élimination des acides aminés résiduels à terminal N, alors que l'analogue de polypeptide contient un acide aminé résiduel ou une séquence de ceux-ci qui bloque l'action d'une aminopeptide située ailleurs qu'à l'extrémité terminale N de l'analogue de polypeptide. Un mode préféré de rálisation comprend l'élimination de la méthionine résiduelle à terminal N et la leucine résiduelle adjacente d'un analogue d'hormone de croissance produit dans des bactéries en mettant en contact l'analogue d'hormone de croissance avec de l'aminopeptidase $i(aeromonas) dans des conditions appropriées permettant l'élimination de la méthionine à terminal N et de la leucine résiduelles. L'invention porte également sur les analogues de polypeptides eucaryotes obtenus par ce procédé.
Latest bibliographic data on file with the International Bureau