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1. (WO1985004671) METHOD OF PRODUCTION OF QUINOPROTEINS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1985/004671 International Application No.: PCT/GB1985/000156
Publication Date: 24.10.1985 International Filing Date: 10.04.1985
IPC:
C12N 9/02 (2006.01) ,C12N 9/04 (2006.01) ,C12Q 1/00 (2006.01) ,G01N 27/30 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
04
acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26
by investigating electrochemical variables; by using electrolysis or electrophoresis
28
Electrolytic cell components
30
Electrodes, e.g. test electrodes; Half-cells
Applicants:
GENETICS INTERNATIONAL INC. [US/GB]; 11 Nuffield Way Abingdon, Oxfordshire OX14 1RL, GB (AllExceptUS)
DUINE, Johanis, Adriaan [NL/NL]; NL (UsOnly)
Inventors:
DUINE, Johanis, Adriaan; NL
Agent:
CLIFFORD, Frederick, Alan; Marks & Clerk 57/60 Lincoln's Inn Fields London WC2A 3LS, GB
Priority Data:
840920810.04.1984GB
Title (EN) METHOD OF PRODUCTION OF QUINOPROTEINS
(FR) PROCEDE DE PRODUCTION DE QUINOPROTEINES
Abstract:
(EN) Method of production of a quinoprotein dehydrogenase from a suitable cell culture, characterised in that, the production of the said quinoprotein is stimulated by the treatment of the said culture with a stimulator, and wherein the stimulator is an alkane and in that said culture is grown in the presence of a non-ionic detergent. The specification further discloses a method for the production of a quinoprotein from a suitable gram-negative bacterial cell culture, characterised in that said culture is grown in the presence of a non-ionic detergent. The methods of the present invention enable these enzymes to be manufactured in gram quantities in a relatively pure form.
(FR) Procédé de production d'une quinoprotéine déshydrogénase à partir d'une culture cellulaire adéquate, dans lequel la production de la quinoprotéine est stimulée par le traitement de ladite culture à l'aide d'un stimulateur, constitué par une alcane, et la culture est cultivée en présence d'un détergent non ionique. Est également décrit un procédé de production d'une quinoprotéine à partir d'une culture cellulaire adéquate de bactéries gram-négatives, cette culture étant cultivée en présence d'un détergent non ionique. Les procédés ci-décrits permettent la production de ces enzymes en quantités gram et sous une forme relativement pure.
Designated States: US
European Patent Office (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0179789