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1. (WO1985004663) METHOD OF DETECTING NUCLEIC ACID SEQUENCES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1985/004663 International Application No.: PCT/US1984/000525
Publication Date: 24.10.1985 International Filing Date: 06.04.1984
IPC:
C12N 15/64 (2006.01) ,C12Q 1/68 (2006.01) ,C12Q 1/70 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
64
General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
70
involving virus or bacteriophage
Applicants:
LIFE TECHNOLOGIES INC. [US/US]; P.O. Box 6009 Gaithersburg, MD 20877, US (AllExceptUS)
HARTLEY, James, L. [US/US]; US (UsOnly)
BERNINGER, Mark, S. [US/US]; US (UsOnly)
Inventors:
HARTLEY, James, L.; US
BERNINGER, Mark, S.; US
Agent:
HOCHBERG, Peter, D.; Woodling, Krost, Rust and Hochberg 655 Huntington Building Cleveland, OH 44115-1482, US
Priority Data:
Title (EN) METHOD OF DETECTING NUCLEIC ACID SEQUENCES
(FR) PROCEDE DE DETECTION DE SEQUENCES D'ACIDES NUCLEIQUES
Abstract:
(EN) A process for detecting specific nucleotide sequences, called targets, in which a special DNA probe molecule, called a probe-vector, is capable of transforming bacteria if and only if it is held in a circular configuration by base pairing to a target nucleic acid, said transformation resulting in the detection of a phenotype specified by the probe-vector, said detection establishing the presence, absence, or quantity of the target; and a probe-vector molecule for performing the process.
(FR) Procédé de détection de séquences de nucléotides spécifiques appelées cibles, où une molécule-sonde spéciale d'ADN appelée vecteur-sonde, est capable de transformer la bactérie si et seulement si elle est maintenue selon une configuration circulaire par appariage des bases à un acide nucléique cible, ladite transformation résultant en la détection d'un phénotype spécifié par le vecteur-sonde, ladite détection établissant la présence, l'absence ou la quantité de la cible. Est également décrite une molécule de vecteur-sonde permettant de réaliser le présent procédé.
Designated States: JP, US
European Patent Office (AT, BE, CH, DE, FR, GB, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0175689US4808519DE000000175689DE000003485834