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1. (WO1985001580) IMMUNOASSAY FOR SERUM ANTIBODIES WITH LIPOSOME REAGENT HAVING DESTABILIZATION COMPONENT

Pub. No.:    WO/1985/001580    International Application No.:    PCT/US1984/001541
Publication Date: Apr 11, 1985 International Filing Date: Sep 26, 1984
IPC: G01N 33/554
Applicants: THE LIPOSOME COMPANY, INC.
Inventors: JANOFF, Andrew, Stuart
OSTRO, Marc, Jeffrey
WEINER, Alan, Lee
WEISSMANN, Gerald
SIEBOLD, James, R.
Title: IMMUNOASSAY FOR SERUM ANTIBODIES WITH LIPOSOME REAGENT HAVING DESTABILIZATION COMPONENT
Abstract:
An immunoassay utilizing a novel liposome composition in which there is incorporated a stabilizing or destabilizing component and an antigen. The presence of cognate antibodies in test samples is detected by the alteration of the supramolecular structure of the liposomes resulting in changes in stabilization. Destabilization can be detected, and in certain cases, caused by, the addition of magnesium or calcium ions. Increased stabilization can be detected by the polymerization of bilayer components in response to ultraviolet light. Antibody-antigen interactions at the liposome surface mediate the stabilization/destabilization response.