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1. WO1985000185 - CLONING VECTOR, METHOD OF CONSTRUCTING SUCH, AND METHOD OF CONCENTRATING AND PURIFYING PRODUCT PROTEINS PRODUCED BY THE CLONING VECTOR

Publication Number WO/1985/000185
Publication Date 17.01.1985
International Application No. PCT/US1984/000491
International Filing Date 30.03.1984
IPC
C12N 15/62 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
62DNA sequences coding for fusion proteins
C12N 15/70 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
C12N 15/75 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75for Bacillus
CPC
C07K 2319/035
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
01containing a localisation/targetting motif
035containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
C07K 2319/61
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
61containing an enzyme fusion for detection (lacZ, luciferase)
C12N 15/625
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
62DNA sequences coding for fusion proteins
625containing a sequence coding for a signal sequence
C12N 15/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
C12N 15/75
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75for Bacillus
Applicants
  • BIOVEC TECHNOLOGY, INC. [US]/[US]
Inventors
  • WILLIAMS, Gordon, L.
Agents
  • MIELIULIS, Benjamin @
Priority Data
508,39129.06.1983US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CLONING VECTOR, METHOD OF CONSTRUCTING SUCH, AND METHOD OF CONCENTRATING AND PURIFYING PRODUCT PROTEINS PRODUCED BY THE CLONING VECTOR
(FR) VECTEUR DE CLONAGE, SON PROCEDE DE PREPARATION, AINSI QU'UN PROCEDE POUR CONCENTRER ET PURIFIER DES PROTEINES DE PRODUITS PRODUITES PAR LE VECTEUR DE CLONAGE
Abstract
(EN)
A novel cloning vector characterized by inverted repeated DNA sequences between which is inserted an active replicative origin. Use of the cloning vector, when introduced into a host cell, produces foreign product protein whose signal sequence is derived from other than the host cell, the signal sequence mediating membrane transport of the product protein but retaining it bound to the cell surface until released by a change in the environmental condition surrounding the host cells. The system provides a means of controlling product protein release for concentration in highly purified form.
(FR)
Nouveau vecteur de clonage caractérisé par des séquences d'ADN inversées répétées entre lesquelles est insérée une origine de duplication active. L'utilisation dudit vecteur de clonage, lorsqu'il est introduit dans une cellule hôte, produit une protéine de produit étrangère dont la séquence signal est dérivée d'un point de départ autre que la cellule hôte, la séquence signal servant de médiateur pour le transport membranaire de la protéine de produit mais retenant celle-ci liée à la surface cellulaire jusqu'à libération par une modification des conditions ambiantes dans lesquelles se trouvent les cellules hôtes. Ce système offre un moyen de régulation de la libération de protéines de produit en vue d'une concentration sous une forme très purifiée.
Also published as
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