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Machine translation
1. (WO1982000058) PROCESSES AND APPARATUS FOR CARRYING OUT SPECIFIC BINDING ASSAYS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1982/000058    International Application No.:    PCT/GB1981/000107
Publication Date: 07.01.1982 International Filing Date: 22.06.1981
IPC:
G01N 33/543 (2006.01), G01N 33/577 (2006.01)
Applicants: UNILEVER PLC (GB only).
UNILEVER NV (JP only).
DAVIS, Paul, James; (For US Only).
PORTER, Philip; (For US Only)
Inventors: DAVIS, Paul, James; .
PORTER, Philip;
Priority Data:
Title (EN) PROCESSES AND APPARATUS FOR CARRYING OUT SPECIFIC BINDING ASSAYS
(FR) PROCEDES ET APPAREIL POUR EFFECTUER DES TESTS SPECIFIQUES DE LIAISON
Abstract: front page image
(EN)A process for carrying out a specific binding assay (for example an immunoassay) in which (a) a sample under assay, possibly containing a substance being tested for, is reacted with (b) a specific binding partner for the substance being tested for, immobilised on a solid support, and (c) a specific binding partner for the substance being tested for which is conjugated to a detectable marker, thereby to form a complex by reaction between whatever quantities are present of the substance being tested for with reagents (b) and (c), in which the marker is immobilised to the support via the substance being tested for, and is detected or assayed as an index of the quantity present in the sample (a) of any of the substance being tested for; characterised in that reaction ingredients (a), (b) and (c) are all mixed in a single step for reaction in a single reaction liquid, and competitive interference between the binding reactious of the substance being tested for and reagents (b) and (c) is avoided either by use of an antibody of narrow and different, non-interfering specificity, such as a monoclonal antibody, to avoid the interference, or by use of a slow-release form of reagent (c). The apparatus for carrying out the assays has a handling piece (13) with several displacers (11) on which specific binding agents are immobilised.
(FR)Dans un procede d'analyse specifique de liaisons (par exemple un immuno-essai) on fait reagir (a) un echantillon sous analyse, contenant eventuellement une substance a determiner, avec (b) un partenaire specifique de liaison pour la substance a determiner, immobilise sur un support solide, et (c) un partenaire specifique de liaison pour la substance a determiner qui est conjuguee a un marqueur detectable, de maniere a former un complexe reactionnel entre des quantites quelconques presentes de la substance analysee avec des agents de reaction (b) et (c), ou le marqueur est immobilise sur le support par la substance analysee, et il est detecte sous forme d'un indice de la quantite presente dans l'echantillon (a) de toutes substances analysees; il se caracterise en ce que des ingredients de reaction (a), (b) et (c) sont tous melanges dans une seule phase pour reagir dans un seul liquide de reaction, et les interferences competitives entre les reactions de liaison de la substance analysee et les agents de reaction (b) et (c) sont eliminees soit en utilisant un anticorps d'une specificite etroite et differente, sans interference, tel qu'un anticorps monoclonal, pour eviter l'interference, soit en utilisant une forme d'agent de reaction (c) a liberation lente. L'appareil permettant d'effectuer les tests possede une piece de manipulation (13) avec plusieurs organes de deplacement (11) sur lesquels les agents specifiques de liaison sont immobilises.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)