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Machine translation
1. (WO1982000057) INSTANTANEOUS RATE KINETIC METHOD FOR DETERMINING TOTAL PROTEIN IN CEREBROSPINAL FLUID
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1982/000057    International Application No.:    PCT/US1981/000805
Publication Date: 07.01.1982 International Filing Date: 15.06.1981
IPC:
G01N 33/68 (2006.01)
Applicants: BECKMAN INSTRUMENTS, INC.
Inventors:
Priority Data:
Title (EN) INSTANTANEOUS RATE KINETIC METHOD FOR DETERMINING TOTAL PROTEIN IN CEREBROSPINAL FLUID
(FR) PROCEDE CINETIQUE A VITESSE INSTANTANEE POUR DETERMINER LA CONCENTRATION TOTALE EN PROTEINES DANS UN FLUIDE CEREBRO-SPINAL
Abstract: front page image
(EN)An instantaneous rate kinetic method for determining total protein in cerebrospinal fluid which avoids the generally high results of prior art biuret methods. The method comprises the steps of: (a) reacting the total protein present in said cerebrospinal fluid sample with a biuret reagent to form colored reaction products; and (b) spectrophotometrically measuring the instantaneous rate of change of the formation of the colored products. The spectrophotometric measurement is directly proportional to said total protein concentration in the sample being assayed.
(FR)Procede cinetique a vitesse instantanee de determination de la concentration totale en proteines dans un fluide cerebro-spinal permettant d'eliminer les resultats generalement eleves des procedes au biuret de l'art anterieur. Le procede comprend les etapes suivantes: (a) on fait reagir l'ensemble des proteines presentes dans l'echantillon de fluide cerebrospinal avec un agent de reaction au biuret pour former des produits de reaction colores; et (b) on mesure par spectrophotometrie la vitesse instantanee de changement de la formation des produits colores. La mesure spectrophotometrique est directement proportionnelle a la concentration totale en proteines dans l'echantillon analyse.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)