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Machine translation
1. (WO1981002792) DETECTION OF ANTIBIOTICS IN MILK
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1981/002792    International Application No.:    PCT/US1981/000354
Publication Date: 01.10.1981 International Filing Date: 20.03.1981
IPC:
G01N 33/04 (2006.01), G01N 33/94 (2006.01)
Applicants:
Inventors:
Priority Data:
131914 20.03.1980 US
Title (EN) DETECTION OF ANTIBIOTICS IN MILK
(FR) DETECTION D'ANTIBIOTIQUES DANS LE LAIT
Abstract: front page image
(EN)A competitive heterogeneous enzyme immunoassay for detection lactam ring containing antibiotics in milk. The two essential features which permit a more equal competition between the free and conjugate forms of the antibiotic are the use of 1) a purified specific antibody prepared with an immunogen composed of the antibiotic bonded through its lactam ring to a protein and 2) an enzyme antibiotic conjugate formed by the attachment of the enzyme to the antibiotic in a manner which leaves the lactam ring free to compete with antibody. The preparation of the antibody is illustrated in figure 1. The assay scheme is illustrated in figures 4, 7 and 8 The assay is relatively inexpensive and is capable of detecting the antibiotics at a magnitude of 0.01 units per milliliter by relatively untrained personnel.
(FR)Analyse immunologique d'enzymes heterogenes de concurrence pour la detection dans le lait des antibiotiques contenant un cycle lactame. Les deux caracteristiques essentielles qui permettent une concurrence plus equilibree entre les formes libres et conjuguees de l'antibiotique sont l'utilisation de 1) un anticorps specifique purifie prepare avec un immunogene compose de l'antibiotique lie au moyen de son cycle lactame a une proteine et 2) un conjugue enzyme antibiotique forme par la liaison de l'enzyme a l'antibiotique d'une maniere telle que l'anneau de lactame reste libre d'etre en concurrence avec l'anticorps. La preparation de l'anticorps est illustree a la figure 1. Le schema de l'analyse est illustre aux figures 4, 7 et 8. L'analyse est relativement peu couteuse et permet de detecter les antibiotiques a une densite de 0,01 unite par millilitre par un personnel relativement peu entraine.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)