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Machine translation
1. (WO1981001849) PURIFIED HUMAN PROSTATE ANTIGEN
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1981/001849    International Application No.:    PCT/US1980/001708
Publication Date: 09.07.1981 International Filing Date: 23.12.1980
IPC:
A61K 39/00 (2006.01), C07K 16/30 (2006.01)
Applicants:
Inventors:
Priority Data:
108217 28.12.1979 US
Title (EN) PURIFIED HUMAN PROSTATE ANTIGEN
(FR) ANTIGENE PURIFIE DE PROSTATE HUMAIN
Abstract: front page image
(EN)A prostate antigen distinct from prostatic acid phosphatase has been detected in normal, benign hypertrophic and malignant prostatic tissues, but not in other human tissues. The prostate antigen was purified to homogeneity from prostatic tissues by ammonium sulfate precipitation, DEAE-BioGel A anion exchange chromatography, molecular sievings on Sephadex G-100 and Sephadex G-75, and preparative polyacrylamide gel electrophoresis. The purified prostate antigen shows a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing. The molecular weight of purified antigen was estimated by Sephadex G-75 gel filtration to be 33,000 and by sodium dodecyl sulfate polyacrylamide gel electrophoresis to be 34,000 with no subunit. The prostate antigen had an isoelectric point of 6.9.
(FR)Un antigene de prostate different de la phosphatase d'acide prostatique a ete detecte dans des tissus normaux, hypertrophiques benins et prostatiques malins, mais non dans d'autres tissus humains. L'antigene de prostate a ete purifie jusqu'a homogeneite a partir de tissus prostatiques par precipitation de sulfate d'ammonium, chromatographie par echange d'anions DEAE-BioGel A, tamisages moleculaires sur Sephadex G-100 et Sephadex G-75, et electrophorese sur gel de polyacrylamide. L'antigene de prostate purifie presente une seule bande de proteine par electrophorese analytique sur gel de polyacrylamide et par focalisation isoelectrique. Le poids moleculaire de l'antigene purifie a ete estime par filtration sur gel Sephadex G-75 a 33000 et par electrophorese sur gel de polyacrylamide-sulfate de sodium dodecyle a 34000 sans sous-unite. L'antigene de prostate avait un point isoelectrique de 6,9.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)