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Machine translation
1. (WO1981001199) METHOD AND KIT FOR THE CLINICAL SEPARATION OF ALPHA-AND BETA-LIPOPROTEINS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1981/001199    International Application No.:    PCT/EP1980/000113
Publication Date: 30.04.1981 International Filing Date: 16.10.1980
IPC:
G01N 33/92 (2006.01)
Applicants:
Inventors:
Priority Data:
7936573 22.10.1979 GB
Title (EN) METHOD AND KIT FOR THE CLINICAL SEPARATION OF ALPHA-AND BETA-LIPOPROTEINS
(FR) PROCEDE ET DISPOSITIF DE SEPARATION CLINIQUE DE LIPOPROTEINES (ALPHA) ET (BETA)
Abstract: front page image
(EN)Separation method comprising the introduction of a sample of serum or plasma into a microcolumn containing a support which has a sulfated polysaccharide, preferably heparin, covalently bound to it so as to remove from said sample the (Beta)-lipoproteins by binding thereof to this ligand, followed by washing the microcolumn with a first pH buffered solution and collecting the eluant representing a first fraction containing the (Alpha)-lipoprotein portion of the sample. Then the microcolumn is washed with a second pH buffered solution so as to release the ligand-bound (Beta)-lipoproteins and to recover a second fraction containing the (Beta)-lipoprotein portion of the sample. Afterwards, it is possible to separately determine the (Alpha)- and (Beta)-lipoprotein-cholesterol, as well as the respective (Alpha) (Beta) ratios of phospholipids, triglycerides and various apoproteines, and to calculate the respective ratios (Alpha) (Beta), all useful as clinical diagnostic indexes and allowing to obtain a complete lipoprotein profiling. The invention also concerns a kit comprising in a transportable box all the implements and reagents duly prepared so as to easily and quickly carry out the above method.
(FR)Procede de separation comprenant l"introduction d"un echantillon de serum ou de plasma dans une micro-colonne contenant un support qui possede un polysaccharide sulfate, de preference l"heparine, liee par covalence de maniere a extraire dudit echantillon les lipoproteines (Beta) par leur liaison sur ce liant, suivie du lavage de la micro-colonne avec une premiere solution a pH tamponne et recuperation de l"eluant representant une premiere fraction contenant la portion de lipoproteine (Alpha) de l"echantillon. Ensuite la micro-colonne est lavee avec une seconde solution a pH tamponne de maniere a liberer les lipoproteines (Beta) liees au liant et recuperer une seconde fraction contenant la portion de lipoproteine (Beta) de l"echantillon. Ensuite, il est possible de determiner separement les proteines-cholesterol (Alpha) et (Beta) ainsi que les rapports respectifs (Alpha) (Beta) des phospholipides, triglycerides et apoproteines diverses, et de calculer les rapports respectifs (Alpha) (Beta), utiles comme indice diagnostic clinique et permettant d"obtenir un profil complet des lipoproteines. Un ensemble se presentant sous la forme d"une mallette transportable comprend tous les instruments et les agents de reaction correctement prepares de maniere a mettre en oeuvre facilement et rapidement le procede.
Designated States:
Publication Language: German (DE)
Filing Language: German (DE)