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Machine translation
1. (WO1981000913) MICROCHROMATOGRAPHIC DEVICE AND METHOD FOR RAPID DETERMINATION OF A DESIRED SUBSTANCE
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1981/000913    International Application No.:    PCT/US1980/001217
Publication Date: 02.04.1981 International Filing Date: 22.09.1980
IPC:
G01N 30/60 (2006.01), G01N 33/66 (2006.01), G01N 33/72 (2006.01), G01N 30/02 (2006.01), G01N 30/38 (2006.01)
Applicants:
Inventors:
Priority Data:
78145 24.09.1979 US
Title (EN) MICROCHROMATOGRAPHIC DEVICE AND METHOD FOR RAPID DETERMINATION OF A DESIRED SUBSTANCE
(FR) DISPOSITIF MICRO-CHROMATIQUE ET PROCEDE DE DETERMINATION RAPIDE D"UNE SUBSTANCE DESIREE
Abstract: front page image
(EN)A combination of a microcolumn (12) having a packing (16) of a suitable absorbent material and a centrifuge tube, (42). A predetermined volume of an eluent being capable of selectively eluting a desired substance through the microcolumn (12) is contained above the packing (16) of the absorbent material. The microcolumn (12) and the centrifuge tube (42) are dimensioned in such a manner that upon centrifugation of the predetermined volume of the eluent through the microcolumn, a level (46) of the eluent remaining in the microcolumn (12), and a level (44) of the eluent in the centrifuge tube (42) coincide substantially at or above a top surface (18) of the packing (16) of the absorbent material. Consequently centrifugation of the assembly of the microcolumn (12) and the centrifuge tube (42) causes passage of the predetermined volume of eluent through the microcolumn (12). Determination or assay of the selectively eluted desired substance may be performed by spectrophotometric methods. The disclosed device and method is particularly adapted for the determination or assay of a relative concentration of glycosylated hemoglobin species present in the red blood cells of a specific person.
(FR)Une combinaison d"une micro-colonne (12) ayant une garniture (16) d"un materiau approprie absorbant et un tube de centrifugation (42). Un volume predetermine d"un eluant capable d"effectuer selectivement l"elution d"une substance desiree au travers de la micro-colonne (12) est contenu au-dessus de la garniture (16) de materiau absorbant. La micro-colonne (12) et le tube de centrifugation (42) sont dimensionnes de telle maniere que, par centrifugation du volume predetermine de l"eluant au travers de la micro-colonne, un niveau (46) de l"eluant restant dans la micro colonne (12) et un niveau (44) de l"eluant dans le tube centrifugation (42) coincident sensiblement avec ou au-dessus de la surface superieure (18) de la garniture (16) de materiau absorbant. En consequence, la centrifugation de l"ensemble de la micro-colonne (12) et du tube de centrifugation (42) provoque le passage d"un volume predetermine d"eluant au travers de la micro-colonne (12). La determination ou analyse de la substance desiree eluee de maniere selective peut etre effectuee par des procedes de spectrophotometrie. Le dispositif et le procede decrits se pretent particulierement a la determination ou analyse d"une concentration relative d"especes d"hemoglobines glycosylees presentes dans les cellules rouges du sang d"une personne.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)