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Machine translation
1. (WO1980002849) PULSED VOLTAMMETRIC DETECTION OF MICROORGANISMS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1980/002849    International Application No.:    PCT/US1980/000755
Publication Date: 24.12.1980 International Filing Date: 18.06.1980
IPC:
C12M 1/34 (2006.01), C12Q 1/04 (2006.01), G01N 27/416 (2006.01), G01N 33/487 (2006.01)
Applicants:
Inventors:
Priority Data:
49561 18.06.1979 US
Title (EN) PULSED VOLTAMMETRIC DETECTION OF MICROORGANISMS
(FR) DETECTION VOLTAMETRIQUE A IMPULSION DE MICRO ORGANISMES
Abstract: front page image
(EN)Reliable and rapid detection of microorganisms is accomplished in an electroanalytical cell using a pulsed voltammetric detection technique employing the growth medium as the electrolyte and analyte and using simple wire electrodes fabricated from readily available materials. Organism detection occurs as a consequence of the depletion of oxygen in the growth medium/electrolyte caused by aerobic metabolism. Times-to-detection vary with inoculum strength in a predictable fashion, permitting quantification of the organism in question when results are compared to those obtained using known inocula of the same organism. The low duty cycle of the pulsed measurement enables the determination of the relative redox potential in the same cell using the same set of electrodes in order to provide information which may be characteristic of the type of organism being studied.
(FR)Une detection sure et rapide de micro-organismes est effectuee dans une cellule electro-analytique par une technique de detection voltametrique a impulsions utilisant le milieu de croissance comme electrolyte et analyte et utilisant des electrodes en fils metalliques simples fabriquees a partir de materiaux disponibles. La detection de l"organisme resulte l"epuisement d"oxygene dans le milieu/electrolyte de croissance du au metabolisme aerobie. Les temps de detection varient avec la force d"inoculum d"une maniere predite a l"avance, permettant la quantification de l"organisme en question lorsque les resultats sont compares a ceux obtenus en utilisant l"inocula connu du meme organisme. Le cycle de faible rendement de la mesure pulsee permet la determination du potentiel redox relatif dans la meme cellule utilisant le meme jeu d"electrodes de maniere a produire des informations qui peuvent etre caracteristiques du type d"organisme etudie.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)