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Machine translation
1. (WO1980002157) A PROCESS FOR ENZYMATIC PRODUCTION OF PEPTIDES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1980/002157    International Application No.:    PCT/DK1980/000020
Publication Date: 16.10.1980 International Filing Date: 01.04.1980
IPC:
C07K 1/02 (2006.01), C07K 5/06 (2006.01), C07K 5/072 (2006.01), C07K 5/078 (2006.01), C07K 5/083 (2006.01), C07K 5/087 (2006.01), C07K 5/103 (2006.01), C12P 21/02 (2006.01), A61K 38/00 (2006.01)
Applicants:
Inventors:
Priority Data:
1443/79 06.04.1979 DK
Title (EN) A PROCESS FOR ENZYMATIC PRODUCTION OF PEPTIDES
(FR) PROCEDE ENZYMATIQUE DE PREPARATION DE PEPTIDES
Abstract: front page image
(EN)A peptide having the formula A-B wherein A represents an N-terminal protected amino acid residue or an optionally N-terminal protected peptide residue and B represents an optionally C-terminal protected amino acid residue, is prepared by reacting a substrate component selected from the group consisting of (a) amino acid esters, peptide esters and depsipeptides of the formula A-OR1, A-SR1 or A-SeR1 wherein A is as defined above and R1 represents alkyl, aryl, heteroaryl, aralkyl or an (Alpha)-des-amino fragment of an amino acid residue, (b) optionally N-substituted amino acid amides and peptide amides of the formula A-NR2R2" wherein A is as defined above and R2 and R2" represent hydrogen, alkyl, aryl, heteroaryl or aralkyl, and (c) optionally N-terminal protected peptides of the formula A-X wherein A is as defined above and X represents an amino acid; with an amine component selected from the group consisting of (a) amino acids of the formula H-B-OH, (b) optionally N-substituted amino acid amides of the formula H-B-NR3R3" wherein B is an amino acid residue and R3 and R3" represent hydrogen, hydroxy, amino or alkyl, aryl, heteroaryl or aralkyl, and (c) amino acid esters of the formula H-B-OR4, H-B-SR4 or H-B-SeR4 wherein B is an amino acid residue and R4 represents alkyl, aryl, heteroaryl and aralkyl, in the presence of a carboxypeptidase enzyme in an aqueous solution or dispersion having a pH from 5 to 10.5, preferably at a temperature of from 20 to 50 C, to form a peptide, and subsequently cleaving a group R3 or R4 or an N-terminal protective group, if desired.
(FR)Un peptide de formule A - B, dans lequel A represente un acide amine a terminaison N protegee ou un reste de peptide a terminaison N protegee et B un reste d"acide amine a terminaison C protegee, est prepare par la mise en reaction d"un compose substrat choisi parmi le groupe comprenant a) des esters d"acides amines, des esters de peptides et des depsipeptides de formule A-OR1, A-SR1 ou A-SeR1 dans lesquels A est defini comme ci-dessus et R1 represente un groupe alkyle, aryle, heteroaryle, aralkyle ou un fragment (Alpha)-des-amino d"un reste d"acide amine, b) des amides d"acides amines N-substitues et des amides de peptides de formule A-NR2 R2", dans laquelle A est comme defini ci-dessus et R2 et R2" representent un atome d"hydrogene, un groupe alkyle, aryle, heteroaryle ou aralkyle, c) des peptides a terminaison N protegee de formule A - X dans laquelle A est comme defini ci-dessus et X represente un acide amine, avec un composant amine choisi parmi le groupe comprenant a) des acides amines de formule H-B-OH b) des amides d"acides amines N-substitues de formule H-B-NR3R3", dans laquelle B est un reste d"acide amine et R3 et R3" representent un atome d"hydrogene, un groupe hydroxy, amino ou alkyle, aryle, heteroaryle ou aralkyle, et c) des esters d"acides amines de formules H-B,OR4, H-B-SR4 ou H-B -SeR4 dans lesquelles B est un reste d"acide amine et R4 represente un groupe alkyle, aryle, heteroaryle et aralkyle; en presence d"un enzyme carboxypeptidase en solution aqueuse ou en dispersion ayant un pH de 5 a 10,5, de preference a une temperature d"environ 20 a 50 C pour former un peptide, puis l"enlevement d"un groupe R3 ou R4 ou d"un groupe de protection de terminaison N si on le desire.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)