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Pub. No.:    WO/1980/001415    International Application No.:    PCT/US1979/001145
Publication Date: 10.07.1980 International Filing Date: 28.12.1979
A61K 51/08 (2006.01), G01N 33/50 (2006.01), G01N 33/82 (2006.01)
Priority Data:
270 02.01.1979 US
Abstract: front page image
(EN)It has been determined that ingested vitamin B12 is bound by R protein in gastric juice, but that it is not capable of absorption in this form. In a system with a normally functioning exocrine pancreas, R protein is degraded by pancreatic proteases in the small intestine, which then allows the B12 to be transferred and bound to intrinsic factor protein (IF) from which it can be absorbed. This phenomenon serves as the basis for a test for pancreatic exocrine function in which the following compositions are ingested: (1) a complex of IF and B12 in which the B12 is labelled; (2) a combination of another binding material, such as R protein, and B12, in which the B12 is labelled (in preferred embodiments the B12 is labelled differently than the B12 in the IF-B12 complex); and (3) means for preventing R protein in the gastric juices from removing B12 from the IF-B12 complex, (in preferred embodiments such means are B12 analogues that bind to R protein in the gastric juices; but which do not bind to IF). In preferred embodiments of the test, unbound IF is also ingested. As a part of the preferred process, steps are also taken to tie up B12 binding proteins which are normally in the test subject"s blood and plasma. Subsequently, the subject"s excreted urine is measured for the amount of labelled B12 which was originally ingested as IF-B12 and for the amount of B12 originally bound to the other binding material, such as R-B12. The amount of B12 from each source which is excreted in the urine, and the ratio between them, is indicative of the functioning of the subject"s exocrine pancreas. Other aspects of the present invention include compositions containing combinations of test compositions. Yet another aspect of the invention is provided by methods of making several of the test compositions and the resulting products.
(FR)Il a ete determine que la vitamine B12 ingeree est liee par la proteine R dans le suc gastrique, mais qu"elle n"est pas apte a etre absorbee sous cette forme. Dans un systeme ayant un pancreas a fonction exocrine normale, la proteine R est degradee par les proteases pancreatiques dans le petit intestin, ce qui permet alors a la B12 d"etre transferee et liee par la proteine du facteur intrinseque (IF) a partir duquel elle peut etre absorbee. Ce phenomene sert de base a un essai de la fonction exocrine du pancreas dans lequel les compositions suivantes sont ingerees: (1) un complexe de IF et de B12 dans lequel la B12 est marquee; (2) une combinaison d"un autre materiau de liaison, tel que la proteine R, et la B12 dans laquelle la B12 est marquee (dans des mises en oeuvre preferentielles la B12 est marquee differemment de la B12 dans le complexe IF-B12); et (3) des moyens pour eviter que la proteine R des sucs gastriques n"enleve la B12 du complexe IF-B12, (dans des mises en oeuvre preferees, de tels moyens sont des analogues de B12 qui se lient a la proteine R dans les sucs gastriques mais qui ne se lient pas a l"IF). Dans des mises en oeuvre preferentielles de cet essai, de l"IF non lie est egalement ingere. En tant que partie du procede prefere, des procedures sont effectuees pour lier les proteines liantes de la B12 qui sont normalement presentes dans le sang et le plasma de l"organisme a essayer. Ensuite l"urine du patient est analysee pour la quantite de B12 marquee qui avait ete originalement ingeree sous la forme de IF-B12 et pour la quantite de B12 originalement liee a un autre materiel de liaison, tel que la R-B12. La quantite de B12 de chaque source qui est excretee dans l"urine et le rapport entre elles est une indication de la fonction exocrine du pancreas du sujet. D"autres aspects de la presente invention comprennent des compositions contenant les combinaisons des compositions d"essai. Les procedes de preparation de plusieurs des compositions d"essai et les produits en resultant constituent un autre aspect de l"invention.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)