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Machine translation
1. (WO1980000455) COLORIMETRIC IMMUNOASSAY EMPLOYING A CHROMOPROTEIN LABEL
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1980/000455    International Application No.:    PCT/US1979/000609
Publication Date: 20.03.1980 International Filing Date: 15.08.1979
IPC:
G01N 33/539 (2006.01)
Applicants:
Inventors:
Priority Data:
933903 15.08.1978 US
Title (EN) COLORIMETRIC IMMUNOASSAY EMPLOYING A CHROMOPROTEIN LABEL
(FR) IMMUNOESSAI COLORIMETRIQUE UTILISANT UNE CHROMOPROTEINE DE MARQUAGE
Abstract: front page image
(EN)Quantitative and qualitative measurement of antigenic materials present in blood serum or other biological fluids or cells is effected by an extremely precise and sensitive colorimetric immunoassay process. This process comprises adding to the sample to be tested a colorimetric immunoassay agent which comprises an adduct of (1) an antibody for the antigen to be tested and (2) a chromoprotein which is capable of chemically coupling to the antibody by covalent bonding and which chromoprotein imparts to the adduct a characteristic absorbance spectrum in the visible light wavelength range. After reacting this colorimetric immunoassay agent with the antigen to form a reagent-antigen complex, either in the complex-containing fraction or a fraction containing unreacted reagent can be analysed using standard colorimetric equipment and procedures to provide a fast, simple and direct measurement of the antigen concentration in the sample.
(FR)La determination quantitative et qualitative de materiel antigenique present dans le serum sanguin ou autres fluides biologiques ou cellules est realisee par un procede d"immunoessai colorimetrique extremement precis et sensible. Le procede comprend l"addition a l"echantillon a doser d"un agent d"immunoessai colorimetrique qui comprend un corps compose de (1) un anticorps correspondant a l"antigene a doser et (2) une chromo proteine qui est apte a se fixer chimiquement a l"anticorps par liaison covalente et cette chromoproteine communique au corps compose un spectre d"absorption caracteristique dans la zone des longueurs d"onde du visible. Apres avoir fait reagir cet agent d"immunoessai colorimetrique avec l"antigene pour former un complexe reactif-antigene on peut, soit dans la fraction comprenant le complexe, soit dans une fraction comprenant le reactif non transforme, analyser ces fractions en utilisant un equipement colorimetrique conventionnel pour fournir une determination rapide, simple et directe de la concentration en antigene dans l"echantillon.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)