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Machine translation
1. (WO1980000454) METHOD OF MEASURING SUBSTRATE OF XANTHINE OXIDASE,AND COMPOSITION FOR THE DETERMINATION
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1980/000454    International Application No.:    PCT/JP1979/000219
Publication Date: 20.03.1980 International Filing Date: 17.08.1979
IPC:
C12N 9/04 (2006.01), C12Q 1/26 (2006.01)
Applicants:
Inventors:
Priority Data:
78/100784 18.08.1978 JP
Title (EN) METHOD OF MEASURING SUBSTRATE OF XANTHINE OXIDASE,AND COMPOSITION FOR THE DETERMINATION
(FR) METHODE DE MESURE D"UN SUBSTRAT DE XANTHINE OXYDASE ET COMPOSITION POUR SA DETERMINATION
Abstract: front page image
(EN)Substrates of xanthine oxidase such as xanthine, hypoxanthine, guanine, 2-hydroxypyrine, etc., are easily measured by subjecting then to microorganism-originating xanthine oxidase, and then measuring the produced hydrogen peroxide using a hydrogen peroxide-measuring system comprising peroxidase and a color-forming agent. This analysis is conducted in one stage by using an analysis composition comprising the xanthine oxidase and the hydrogen peroxide-measuring system. This method can be applied to measuring a material participating in a system of quantitatively producing the substrate as a result of reaction or decomposition. Where the system involves enzymatic reaction, the material can also be measured in one stage. Xanthine oxidase to be used for this purpose is different from known ones in molecular weight etc., and can be yielded by cultivating microorganism of enterobacter genus.
(FR)Des substrats de xanthine oxydase tels que la xanthine, l"hypoxanthine, la guanine, 2-hydroxypyrine, etc., sont facilement mesures en les soumettant a de la xanthine oxydase donnant naissance a des microorganismes, puis en mesurant l"eau oxygenee produite en utilisant un systeme de mesure d"eau oxygenee comprenant une peroxydase et un agent colorant. Cette analyse est conduite en une etape en utilisant une composition d"analyse comprenant la xanthine oxydase et le systeme de mesure d"eau oxygenee. Cette methode peut etre appliquee pour le dosage d"un materiau faisant partie d"un systeme de production quantitative du substrat resultant d"une reaction ou decomposition. Lorsque le systeme comprend une reaction enzymatique, le materiau peut egalement etre dose en une etape. La xanthine oxydase utilisee a cet effet est differente de celle que l"on connait en poids moleculaire, etc., et peut etre produite en cultivant des microorganismes du genre anterobacterie.
Designated States:
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)