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1. (WO1980000026) INSOLUBILIZED METHYLATED ALBUMIN CONJUGATES FOR AUTOIMMUNE DISEASE FLUOROIMMUNOASSAYS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1980/000026 International Application No.: PCT/US1979/000310
Publication Date: 10.01.1980 International Filing Date: 09.05.1979
IPC:
G01N 33/564 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
564
for pre-existing immune complex or autoimmune disease
Applicants:
Inventors:
Priority Data:
91186502.06.1978US
Title (EN) INSOLUBILIZED METHYLATED ALBUMIN CONJUGATES FOR AUTOIMMUNE DISEASE FLUOROIMMUNOASSAYS
(FR) CONJUGES D"ALBUMINE METHYLES INSOLUBILISES POUR TEST FLUORO-IMMUNO SUR LES MALADIES AUTO-IMMUNES
Abstract:
(EN) A method of detecting antibodies to native deoxyribonucleic acid (n-DNA), anti-nuclear antibodies, antibodies to non-histone containing protein, rheumatoid factors, antibodies to thyroglobulin, and an immunofluorescent assay for such detection is described. The method comprises incubating an insoluble conjugate of methylated bovine serum albumin (mBSA)-native deoxyribonucleic acid (n-DNA) or other substrates with serum from patients with systemic lupus erythematosus (SLE) or other autoimmune diseases for a sufficient length of time, washing the precipitate or flocculate, separating and discarding therefrom the supernatant fluid, adding a fluorescein labeled anti-immunoglobulin antibody tothe washed flocculate and incubating the labeled mixture for a sufficient length of time, washing the incubated flocculate and separating therefrom the supernatant fluid, suspending the pellet in a liquid medium to determine the fluotal rescence of the flocculate, which is proportional to the concentration of antibodies to deoxyribonucleic acid and others in serum specimen. The insoluble conjugate of methylated bovine serum albumin (mBSA)-native deoxyribonucleic acid (n-DNA) retains the double-stranded form of native deoxyribonucleic acid (n-DNA).
(FR) Methode de detection d"anticorps sur de l"acide desoxyribonucleique natif (n-ABN), des anticorps antinucleaires, des anticorps au non-histone contenant des proteines, des facteurs rheumatoides, des anticorps a la tyroglobuline, et un essai immunofluorescent pour une telle detection. La methode consiste a incuber un conjuge insoluble d"albumine de serum bovin methyle (mASB)-acide desoxyribonucleique natif (n-ABN) ou autres substrats avec du serum de patient avec du lupus erythematosus systemique (LES) ou autres maladies autoimmunes pendant une periode de temps suffisamment longue, a laver le precipite ou floculat, a en separer et jeter le fluide de surnageant, a ajouter un anticorps anti-immunoglobuline marque a la fluoresceine au floculat lave et a incuber le melange marque pendant une periode de temps suffisamment longue, a laver le floculat incube et a en separer le fluide surnageant, a mettre en suspension les "pellets" dans un milieu liquide pour determiner la fluorescence du floculat, qui est proportionnel a la concentration d"anticorps a l"acide desoxyribonucleique et autres en specimens de serum. Le conjuge insoluble d"albumine de serum bovin methylee (mASB)-acide desoxyribonucleique (n-ADN) garde la forme a double toron de l"acide desoxyribonucleique natif (n-ADN).
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)