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1. WO1979001169 - STREPTOMYCES PLASMIDS AND THEIR USE AS VECTORS FOR THE INCORPORATION OF NUCLEIC ACID INTO CELLULAR SYSTEMS

Publication Number WO/1979/001169
Publication Date 27.12.1979
International Application No. PCT/GB1979/000095
International Filing Date 01.06.1979
IPC
C12N 15/76 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
76for Actinomyces; for Streptomyces
CPC
C12N 15/76
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
76for Actinomyces; for Streptomyces
Applicants
Inventors
Priority Data
782621901.06.1978GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) STREPTOMYCES PLASMIDS AND THEIR USE AS VECTORS FOR THE INCORPORATION OF NUCLEIC ACID INTO CELLULAR SYSTEMS
(FR) PLASMIDES STREPTOMICES ET LEUR UTILISATION EN TANT QUE VECTEUR POUR L"INCORPORATION D"ACIDES NUCLEIQUES DANS DES SYSTEMES CELLULAIRES
Abstract
(EN)
Novel Streptomyces plasmids have the characteristic that their presence in non-integrated form in a micro-organism of the species Streptomyces lividans confers on that micro-organism the properties (a) of forming "pocks" when grown on a "lawn" of that strain of micro-organism deposited with the National Collection of Industrial Bacteria (NCIB) under the reference number 11416, and (b) of not forming "pocks" when grown on a "lawn" of that strain of micro-organism deposited with the NCIB under the reference number 11417, or which is derivable from a plasmid having such a characteristic by the removal or addition of DNA therefrom. The plasmids are prepared from micro-organisms containing them or by the manipulation of other plasmids of the group and are of value as vectors for the introduction of nucleic acid into microorganisms.
(FR)
De nouveaux plasmides streptomices se caracterisent en ce que leur presence sous forme non integree dans un micro-organisme de l"espece streptomices lividants donnent a ce micro-organisme les proprietes (a) de formation de "pocks" (pustules) lorsqu"ils sont cultives sur une "lawn" (tapis) de cette souche de micro-organisme disposee aupres de la "National Collection of Industrial Bacteria" (NCIB) sous le numero de reference 11416, et (b) de non formation de "pocks" lorsqu"ils sont cultives sur un "lawn" de cette souche de micro-organisme depose aupres du NCIB sous le numero de reference 11417, ou pouvant etre derive d"un plasmide ayant de telles caracteristiques en soustrayant ou ajoutant de l"ADN. Les plasmides sont prepares, a partir de micro-organismes les contenant ou par manipulation d"autres plasmides du groupe et sont utiles en tant que vecteur pour l"introduction d"acides nucleiques dans les micro-organismes.
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