WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO1979000942) APPARATUS AND PROCESS FOR CONTINUOUS FLOW ISOELECTRIC FOCUSING
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1979/000942    International Application No.:    PCT/US1979/000238
Publication Date: 15.11.1979 International Filing Date: 16.04.1979
IPC:
B01D 57/02 (2006.01), G01N 27/447 (2006.01)
Applicants:
Inventors:
Priority Data:
897261 18.04.1978 US
Title (EN) APPARATUS AND PROCESS FOR CONTINUOUS FLOW ISOELECTRIC FOCUSING
(FR) METHODE ET APPAREIL DE REGLAGE ISOELECTRIQUE CONTINU
Abstract: front page image
(EN)A method and apparatus for isoelectric focusing of fluids. In accordance with the disclosed method, the flow of fluids to be processed is established in a first direction. This flow of fluids is streamlined by providing a plurality of permeable microporous membranes (52-60) which define generally parallel channels oriented in the first direction. An electrical potential is applied across the streamlined channels of flowing fluid, and isoelectric focusing is achieved on the fluids during the flow thereof since the membranes (52-60) allow interchange of fluid constituents therebetween while providing the desired streamlining. An approximation of "plug" type flow is achieved within the streamlined channels; i.e., flow having an approximately uniform cross-sectional characteristic. In the preferred embodiment of the method of the invention, a recirculation path is established for each of the streamlined channels, such that the fluid flowing out of each channel is recirculated back to the beginning of the channel. Preferably, this is achieved by pumping (400) the fluids in each of the recirculation paths, and also providing cooling (300) for the fluids during the recirculation thereof. In this manner, a number of "passes" are effected to obtain the desired degree of isoelectric focusing. The cooling, which is performed during the recirculation, serves to minimize problems with dissipation of Joule heat during the isoelectric focusing of the fluids in the streamlined channels. Also, in the preferred embodiment of the invention, the first direction is downward such that the streamlined fluids flow under the influence of gravity to permit gravity equilibrium of fluid levels in the channels across the streamlining membranes (52-60).
(FR)Methode et appareil de reglage isoelectrique des fluides. Selon la methode de l"invention, le debit du fluide a traiter est etabli dans une premiere direction. Ce debit de fluide est profile a l"aide d"une pluralite de membranes microporeuses permeables (52-60) qui definissent des canaux paralleles orientes dans la premiere direction. Une tension electrique est appliquee sur les canaux profiles de fluide en ecoulement, et le reglage isoelectrique des fluides est effectue pendant leur debit, les membranes permettant entre elles l"echange des composants des fluides tout en produisant le profilage desire. On obtient une approximation du debit du type "bouchon" dans les canaux profiles. c"est-a-dire un debit ayant des caracte de section de passage approximativement uniforme. Dans le mode preferentiel de la methode selon l"invention, un passage de recirculation est etabli pour chacun des canaux profiles, de sorte que le fluide sortant de chaque canal est remis en circulation au debut du canal. De preference, ceci est effectue par pompage (400) des fluides dans chacun des passages de recirculation et par refroidissement (300) des fluides pendant leur recirculation. De cette maniere, un nombre de "passes" sont effectuees pour obtenir le degre voulu de reglage isoelectrique. Le refroidissement, qui a lieu pendant la recirculation, sert a reduire au minimum les problemes de dissipation de la chaleur par effet Joule pendant le reglage isoelectrique des fluides dans les canaux profiles. Egalement, dans le mode d"execution prefe de l"invention, la premiere direction est vers le bas de sorte que les fluides s"ecoulent sous l"influence de la pesanteur, pour permettre l"equilibre de gravite des niveaux du fluide dans les canaux a travers les membranes (52-60).
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)