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Pub. No.:    WO/1979/000155    International Application No.:    PCT/US1978/000080
Publication Date: 05.04.1979 International Filing Date: 12.09.1978
A01N 25/28 (2006.01), A01N 63/00 (2006.01)
Priority Data:
835817 22.09.1977 US
Abstract: front page image
(EN)An improved insecticidal composition is disclosed comprising a microbial agent of viral or bacterial origin embedded in a microbead, wherein the microbead contains sufficient radiation-absorbing material to effectively shield the agent from sunlight-induced inactivation. Typically the microbead is comprised of a nucleic acid and a protein-containing material, and is stabilized by chemical crosslinking. A method for preparing a microbial insecticide composition is also disclosed comprising: mixing a buffered aqueous solution containing a microbial insect pathogen with an aqueous solution containing a nucleic acid, and mixing the said mixture with an aqueous solution containing a proteinaceous material, thereby spontaneously forming microbeads having the pathogen embedded therein. Typically the microbeads are subsequently stabilized by treatment with chemical crosslinking agents, and typically, prior to the two mixing steps, the microbial insect pathogen is cleaned by washing and its surface charge is modified by the addition of a protein-modifying agent adapted to provide a more uniform surface charge.
(FR)Compose insecticide perfectionne comprenant un agent microbien d"origine virale ou bacterienne noye dans une microcapsule, laquelle contient suffisamment de substance absorbant les radiations pour proteger efficacement l"agent de l"inactivation induite par la lumiere solaire. La microcapsule se caracterise en ce qu"elle comprend un acide nucleique et une substance contenant des proteines et en ce qu"elle est stabilisee par retification. L"invention concerne egalement une methode de preparation du compose insecticide microbien consistant a: melanger une solution aqueuse tamponnee contenant un pathogene d"insecte microbien avec une solution aqueuse contenant un acide nucleique, et a melanger ledit melange avec une solution aqueuse contenant une substance proteique, formant ainsi spontanement des microcapsules contenant le microbe pathogene. Les microcapsules se caracterisent en ce qu"elles sont, par la suite, stabilisees par traitement avec des agents de reticulation et, de maniere caracteristique et avant les deux etapes de melange, le pathogene d"insecte microbien est nettoye par lavage et sa charge en surface est modifiee en ajoutant un agent de modification proteique adapte pour assurer une charge en surface plus uniforme.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)