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1. (WO1979000045) METHOD FOR THE DETERMINATION OF ENZYMES BY DIFFUSION IN A POROUS MATRIX OR BY ELECTROPHORESIS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1979/000045 International Application No.: PCT/SE1978/000020
Publication Date: 08.02.1979 International Filing Date: 13.07.1978
IPC:
C12Q 1/00 (2006.01) ,G01N 27/447 (2006.01) ,G01N 33/558 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26
by investigating electrochemical variables; by using electrolysis or electrophoresis
416
Systems
447
using electrophoresis
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
558
using diffusion or migration of antigen or antibody
Applicants:
Inventors:
Priority Data:
770817914.07.1977SE
Title (EN) METHOD FOR THE DETERMINATION OF ENZYMES BY DIFFUSION IN A POROUS MATRIX OR BY ELECTROPHORESIS
(FR) PROCEDE DE DETERMINATION DE L"ACTIVITE ENZYMATIQUE PAR DIFFUSION DANS UNE MATRICE POREUSE OU PAR ELECTROPHORESE
Abstract:
(EN) Method for the determination of enzymatic activity in which a sample is added to a basin in or a defined surface of a liquid-saturated porous matrix covering a thin layer of a substance sensitive to enzymatic lysis deposited on the solid surface of a carrier. The sample is permitted to diffuse or migrate electrophoretically in the matrix and react with the substance sensitive to enzymatic lysis. The matrix is then removed whereon lysis can be indicated on the solid surface. The enzym-containing sample can be a bacteria colony on the matrix. Plastic material is preferred for the solid surface.
(FR) Procede de determination de l"activite enzymatique dans lequel un echantillon est dispose dans une cuvette formee par une matrice poreuse saturee de liquide, ou sur une surface determinee de cette matrice, ladite matrice poreuse recouvrant une fine couche d"une substance susceptible de subir une lyse par voie enzymatique, deposee sur la surface solide d"un support. On laisse l"echantillon diffuser ou migrer par electrophorese dans la matrice et reagir avec la substance susceptible de lyse par voie enzymatique. On enleve ensuite la matrice et le phenomene de lyse peut etre revele sur la surface solide. L"echantillon contenant l"enzyme peut etre une colonie bacterienne sur la matrice. La surface solide est, de preference, celle d"un support de matiere plastique.
Designated States:
Publication Language: English (EN)
Filing Language: English (EN)