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1. (WO1910000591) FLUORESCENT CYCLIC PEPTIDES, METHODS FOR THE PREPARATION THEREOF, AND USE OF SAID PEPTIDES FOR MEASURING THE ENZYMATIC ACTIVITY OF A PROTEASE ENZYME
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/000591 International Application No.: PCT/EP2009/057194
Publication Date: 07.01.2010 International Filing Date: 10.06.2009
IPC:
C07K 1/10 (2006.01) ,C07K 7/64 (2006.01) ,C12Q 1/37 (2006.01) ,G01N 33/52 (2006.01) ,G01N 33/58 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
10
using coupling agents
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
7
Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
64
Cyclic peptides containing only normal peptide links
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
37
involving peptidase or proteinase
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52
Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58
involving labelled substances
Applicants:
COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES [FR/FR]; 25 rue Leblanc Bâtiment "Le Ponant D" F-75015 Paris, FR (AllExceptUS)
UNIVERSITE VICTOR SEGALEN - BORDEAUX 2 [FR/FR]; 146 rue Léon Saignat F-33076 Bordeaux, FR (AllExceptUS)
BERTHELOT, Thomas [FR/FR]; FR (UsOnly)
DELERIS, Gérard [FR/FR]; FR (UsOnly)
Inventors:
BERTHELOT, Thomas; FR
DELERIS, Gérard; FR
Agent:
ILGART, Jean-Christophe; Brevalex 3, rue du Docteur Lancereaux F-75008 Paris, FR
Priority Data:
085385010.06.2008FR
Title (EN) FLUORESCENT CYCLIC PEPTIDES, METHODS FOR THE PREPARATION THEREOF, AND USE OF SAID PEPTIDES FOR MEASURING THE ENZYMATIC ACTIVITY OF A PROTEASE ENZYME
(FR) PEPTIDES CYCLIQUES FLUORESCENTS, PROCEDES DE PREPARATION DE CEUX-CI ET UTILISATION DE CES PEPTIDES POUR MESURER L'ACTIVITE ENZYMATIQUE D'UNE ENZYME PROTEASE
Abstract:
(EN) The invention relates to a cyclic peptide comprising the following sequence: -S1-X1-S2-X2- wherein S1 is a first target peptide sequence of a protease enzyme E1, S2 is a second target peptide sequence of a protease enzyme E2, S1 and S2 can be the same or different, the first peptide sequence S1 and the second peptide sequence S2 comprising between 4 and 14 amino acids, E1 and E2 corresponding to the same protease enzyme or to two separate protease enzymes, X1 is a probe carrying a fluorescent donor group, and X2 is a probe carrying a fluorescent or non-fluorescent acceptor group. Said peptides can be used in determining the activity of protease enzymes.
(FR) L' invention a trait à un peptide cyclique comprenant la séquence suivante : -S1-X1-S2-X2- dans laquelle S1 est une première séquence peptidique cible d'une enzyme protéase E1, S2 est une seconde séquence peptidique cible d'une enzyme protéase E2, S1 et S2 pouvant être identiques ou différents, ladite première séquence peptidique S1 et ladite seconde séquence peptidique S2 comprenant de 4 à 14 acides aminés, E1 et E2 pouvant correspondre à la même enzyme protéase ou à deux enzymes protéases distinctes, X1 est une sonde porteuse d'un groupe donneur fluorescent et X2 est une sonde porteuse d'un groupe accepteur fluorescent ou non fluorescent. Application de ces peptides à la détermination de l'activité d'enzymes protéases.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: French (FR)
Filing Language: French (FR)