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1. US20120052570 - Method for controlling the biological activity of a protein in a vertebrate cell

Office United States of America
Application Number 13145752
Application Date 22.01.2010
Publication Number 20120052570
Publication Date 01.03.2012
Grant Number 09273115
Grant Date 01.03.2016
Publication Kind B2
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
72
for hormones
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
90
Isomerases (5.)
C12N 15/00
C07K 14/72
C12N 9/90
CPC
C07K 14/721
C07K 2319/715
C12N 9/90
Applicants Daniel Metzger
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
Pierre Chambon
THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
Huimin Zhao
UNIVERSITE DE STRASBOURG
John Katzenellenbogen
Inventors Daniel Metzger
Pierre Chambon
Huimin Zhao
John Katzenellenbogen
Agents Birch, Stewart, Kolasch & Birch, LLP
Title
(EN) Method for controlling the biological activity of a protein in a vertebrate cell
Abstract
(EN)

The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor α, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site-specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.

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