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1. US20090015912 - Total Internal Reflectance Fluorescence (TIRF) Microscope

Office
United States of America
Application Number 11718546
Application Date 08.11.2005
Publication Number 20090015912
Publication Date 15.01.2009
Publication Kind A1
IPC
G02B 21/06
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
06Means for illuminating specimen
G02B 21/08
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
06Means for illuminating specimen
08Condensers
G02B 21/16
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
16adapted for ultra-violet illumination
Applicants Imperial Inovations Limited
Inventors Ferenczi Michael Alan
Agents STERNE, KESSLER, GOLDSTEIN &; FOX P.L.L.C.
Priority Data 0424652 08.11.2004 GB
Title
(EN) Total Internal Reflectance Fluorescence (TIRF) Microscope
Abstract
(EN)

A total internal reflection fluorescent (TIRF) microscope has a conjugate lens (30) which is positioned off of the primary microscope axis (AA). The lens (30) is held by a mount (60) allowing the radial spacing between the microscope axis (AA) and the conjugate lens axis (BB) to be varied. The mount (60) also allows the lens to be rotated around the microscope axis. As the lens (30) rotates, the spot of light (64) created by the incident laser beam moves around the periphery of the objective lens (34). This changes the direction of polarisation of the evanescent wave within which the sample (44) sits. By studying the emitted fluorescence as a function of lens angle, information is provided about the spatial attitude of a molecule under study.