US20080115593 Method for Detecting Conformational Change of Calmodulin, a Method for Screening a Substance Having an Activity that Affects to Conformational Change of Calmodulin

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United States of America

Application Number 11666077

Application Date 07.11.2005

Publication Number 20080115593

Publication Date 22.05.2008

Publication Kind A1

IPC

Applicants FUENCE CO., LTD.

Inventors Mogami Kaname
Kase Hiroshi

Agents OLIFF &; BERRIDGE, PLC

Priority Data 2004325005 09.11.2004 JP

Title

(EN) Method for Detecting Conformational Change of Calmodulin, a Method for Screening a Substance Having an Activity that Affects to Conformational Change of Calmodulin

Abstract

(EN)

The object of this invention is to provide a method for detecting conformational change of calmodulin easily and simply in a short period, quantitatively and in real-time manner, and to provide a method for screening a substance having an activity that affects to conformational change of calmodulin efficiently among numerous substances. To solve the object, a method is provided and the method comprising; subjecting a test sample to be tested for its activity to a sample film comprising calmodulin, detecting change(s) in tension and/or elasticity of the sample film in comparison with prior to subjecting said test sample using a mechanochemical sensor, thereby it enables real-time and short time measurement of the activity of said test sample to induce or to inhibit conformational change of calmodulin.

G	PHYSICS
01	MEASURING; TESTING
L	MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
1	Measuring force or stress, in general
04	by measuring elastic deformation of gauges, e.g. of springs

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
48	involving transferase

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor

G	PHYSICS
01	MEASURING; TESTING
L	MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
1	Measuring force or stress, in general
04	by measuring elastic deformation of gauges, e.g. of springs

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
48	involving transferase

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1. US20080115593 - Method for Detecting Conformational Change of Calmodulin, a Method for Screening a Substance Having an Activity that Affects to Conformational Change of Calmodulin