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1. US20080199924 - Production of degummed fatty acid alkyl esters using both lipase and phospholipase in a reaction mixture

Office
United States of America
Application Number 11916052
Application Date 13.06.2006
Publication Number 20080199924
Publication Date 21.08.2008
Grant Number 08012724
Grant Date 06.09.2011
Publication Kind B2
IPC
C12P 7/62
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
62Carboxylic acid esters
Applicants Novozymes A/S
Inventors Holm Hans Christian
Nielsen Per Munk
Christensen Morten Wurtz
Agents McNamara Kristin J.
Priority Data 2005 00858 13.06.2005 DK
Title
(EN) Production of degummed fatty acid alkyl esters using both lipase and phospholipase in a reaction mixture
Abstract
(EN)

The present invention relates to a method for producing fatty acid alkyl esters, such as fatty acid methyl esters (FAME) and fatty acid ethyl esters with a low level of impurities such as phospholipids. The method of the invention is simplified by combining two process steps into one single process step and is therefore economically cheaper. The method includes mixing water, alcohol, triglyceride and/or free fatty acids a lipolytic enzyme and a phospholipase. Subsequently the aqueous phase, which contains glycerine, residual enzyme and most of the hydrolyzed phospholipids, is separated from the non-aqueous phase, whereby the content of phospholipids in the non-aqueous phase is reduced.