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1. US20070178450 - Method and apparatus for determining level of microorganisms using bacteriophage

Office
United States of America
Application Number 11698673
Application Date 25.01.2007
Publication Number 20070178450
Publication Date 02.08.2007
Publication Kind A1
IPC
C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
Applicants MicroPhage (TM) Incorporation
Inventors Wheeler John H.
Rees Jon C.
Gaisford Gregory S.
Agents PATTON BOGGS LLP
Priority Data 60762749 27.01.2006 US
60794652 24.04.2006 US
60800922 15.05.2006 US
Title
(EN) Method and apparatus for determining level of microorganisms using bacteriophage
Abstract
(EN)

A predetermined amount of parent bacteriophage capable of infecting a target microorganism is added to a sample to create a bacteriophage-exposed sample; the sample is incubated for a defined incubation time and assayed to determine the level of a bacteriophage or bacterial marker in the sample; and if the measured marker level has increased, then the initial concentration of the microorganism exceeds a specific threshold value. An antibiotic in different concentrations is added to different and separate portions of the sample and tested to determine if the bacteriophage marker is present and thereby determine the Minimum Inhibitory Concentration (MIC) of a given antibiotic. The antibiotic preferably is an antibiotic that inhibits DNA replication or protein synthesis.

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