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1. US20070269870 - Methods for assembly of high fidelity synthetic polynucleotides

Office
United States of America
Application Number 11254250
Application Date 18.10.2005
Publication Number 20070269870
Publication Date 22.11.2007
Publication Kind A1
IPC
C12P 19/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Applicants CHURCH GEORGE
AFEYAN NOUBAR
JACOBSON JOSEPH
BAYNES BRIAN M
NESMITH KENNETH G
CHAPMAN BRAD A
STRACK-LOGUE BETTINA
Inventors Church George
Afeyan Noubar
Jacobson Joseph
Baynes Brian M.
Nesmith Kenneth Gabriel
Chapman Brad Alan
Strack-Logue Bettina
Agents ROPES &; GRAY LLP;PATENT DOCKETING 39/41
Priority Data 11067812 28.02.2005 US
11068321 28.02.2005 US
60619650 18.10.2004 US
60643813 13.01.2005 US
60657014 28.02.2005 US
60698560 12.07.2005 US
60727205 14.10.2005 US
Title
(EN) Methods for assembly of high fidelity synthetic polynucleotides
Abstract
(EN)

Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of nucleic acid polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or more oligonucleotides using “universal” primers, reduction of the error rate in oligonucleotide and/or nucleic acid products, and sequence optimization and oligonucleotides design. Also provided are low-purity arrays of nucleic acids and methods for assembling nucleic acids using oligonucleotides obtained from low-purity arrays.