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1. US20060053516 - Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS

Office United States of America
Application Number 11004399
Application Date 03.12.2004
Publication Number 20060053516
Publication Date 09.03.2006
Publication Kind A1
IPC
A61K 39/12
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
12Viral antigens
A01H 1/00
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
A61K 36/18
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
36Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
18Magnoliophyta (angiosperms)
C12N 5/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
04Plant cells or tissues
C12N 15/82
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
Applicants The University of Hong Kong
Inventors Chye Mee Len
Li Hong Ye
Ramalingam Sathiskumar
Poon Leo Lit Man
Peiris Joseph Sriyal Malik
Agents COOPER &; DUNHAM, LLP
Priority Data 11004399 03.12.2004 US
Title
(EN) Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS
Abstract
(EN)

The invention relates to genetically modified plants and progeny thereof which constitute edible plant-derived mucosal vaccines and injectable plant-derived mucosal vaccines against Severe Acute Respiratory Syndrome (SARS). The invention relates to a recombinant vector that transforms specifically, but not limited to, the nuclei and/or plastids of tobacco, tomato and lettuce plants for antigen production. In specific embodiments, the plastid transformation vector expressing the nucleotide sequences are pCV1, pCV6 and pCV8, and their derivatives containing an S1 with nucleotide (but not amino acid) changes to optimize codon usage for expression in plants. The present invention relates to methods of making the modified plants which comprises transformation of plants with vectors for nuclear expression and/or plastid expression of nucleotide sequences of the SARS-CoV virus, fragments, derivatives, analogs, or variants thereof. The present invention also relates to methods of immunization against SARS and methods of antibody detection using the SARS-CoV antigens generated by the plastid and/or nuclear vector(s) transformed plants.