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1. US6472516 - Progestin-regulated gene

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What is claimed is:

1. An isolated DNA molecule comprising a nucleotide sequence more than 80% homologous to any one of the nucleotide sequences shown at:
(i) FIG. 2B (SEQ ID NO:1) from nucleotide 1 to 3018, and
(ii) FIG. 2B (SEQ ID NO:1) from nucleotide 141 to 3018,
wherein said DNA molecule encodes a protein with phosphofructo-2-kinase/fructose-2, 6-biphosphatase activity.
 
2. A DNA molecule according to claim 1, said DNA molecule being of human origin.
 
3. The DNA molecule according to claim 2, said DNA molecule being of human kidney cell or breast cancer cell origin or which encodes a protein normally expressed in human kidney cells, breast tissue or tumor cells.
 
4. An expression vector comprising a DNA molecule according to claim 1.
 
5. A recombinant host cell including a DNA molecule according to claim 1.
 
6. An isolated DNA molecule comprising a progestin-regulatory element(s)(PRE) present in the DNA molecule according to claim 1.
 
7. An isolated DNA molecule comprising a nucleotide sequence more than 90% homologous to any one of the nucleotide sequences shown at:
(i) FIG. 2B (SEQ ID NO:1) from nucleotide 1 to 3018, and
(ii) FIG. 2B (SEQ ID NO:1) from nucleotide 141 to 3018,
wherein said DNA molecule encodes a protein with phosphofructo-2-kinase/fructose-2, 6-biphosphatase activity.
 
8. An isolated DNA molecule having the nucleotide sequence shown at FIG. 2B (SEQ ID NO:1) from nucleotide 1 to 3018, wherein said DNA molecule encodes a protein with phosphofructo-2-kinase/fructose-2, 6-biphosphatase activity or a nucleotide sequence complementary thereto.
 
9. An isolated DNA molecule having the nucleotide sequence shown at FIG. 2B (SEQ ID NO:1) from nucleotide 1 to 470, wherein said DNA molecule encodes a protein with phosphofructo-2-kinase/fructose-2, 6-biphosphatase activity or a nucleotide sequence complementary thereto.
 
10. An isolated DNA molecule having the nucleotide sequence shown at FIG. 2B (SEQ ID NO:1) from nucleotide 141 to 3018, wherein said DNA molecule encodes a protein with phosphofructo-2-kinase/fructose-2, 6-biphosphatase activity or a nucleotide sequence complementary thereto.
 
11. An isolated DNA molecule having the nucleotide sequence shown at FIG. 2B (SEQ ID NO:1) from nucleotide 470 to 2103, wherein said DNA molecule encodes a protein with phosphofructo-2-kinase/fructose-2, 6-biphosphatase activity.
 
12. A polypeptide in a substantially pure form, said polypeptide comprising an amino acid sequence as shown at FIG. 2B (SEQ ID NO:2) or an enzymatic portion thereof, wherein said polypeptide or enzymatic portion thereof has phosphofructo-2-kinase/fructose-2, 6-biphosphatase activity.
 
13. A polypeptide in a substantially pure form, said polypeptide consisting of an amino acid sequence as shown at FIG. 2B (SEQ ID NO:2) wherein said polypeptide or enzymatic portion thereof has phosphofructo-2-kinase/fructose-2, 6-biphosphatase activity.