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1. US20020055186 - Detection of peptides

Office
United States of America
Application Number 09956751
Application Date 19.09.2001
Publication Number 20020055186
Publication Date 09.05.2002
Publication Kind A1
IPC
G01N 33/543
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
C40B 30/04
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
30Methods of screening libraries
04by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
G01N 33/68
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
Applicants Oxford GlycoSciences (UK) Ltd.
Inventors Barry Richard
Platt Albert Edward
Scrivener Elaine
Soloviev Mikhail
Terrett Jonathan Alexander
Agents DARBY &; DARBY P.C.
Priority Data 0022978 19.09.2000 GB
Title
(EN) Detection of peptides
Abstract
(EN)

The present invention provides a method and devices for determining the presence of proteins of interest in a sample. In practice, the method comprises submitting the sample to conditions that allow fragmentation of the proteins into target peptide fragments. The target peptide fragments are then contacted with an array of capture agents, such as antibodies, immobilized on a solid support. The capture agents recognize a target peptide fragment of a protein of interest. Binding of a target peptide fragment with an antibody is indicative of the presence of a protein of interest in the sample. The invention further provides a method for producing an array for capturing a target peptide fragment of a protein of interest, which comprises immobilizing capture agents on a solid support, wherein each capture agent specifically recognizes a sequence of a region of a target peptide fragment from a different protein of interest. The methods and arrays (devices) of the invention provide for proteomics, diagnosis, pharmacoproteomics, identification of markers of disease, and drug target discovery. The methods and arrays are particularly suitable for generating a database of information relating to protein expression.