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There is provided a pulsatile flow pressure system for treating tissue, such as, for example, bone tissue. This system generally includes one, and preferably two, pressure chambers configured to receive the natural ends of intact bone. Alternate positive and negative pressures are enacted on the bone ends to force fluid into the bone and substantially out through an exterior surface thereof. Alternatively, both ends of an intact bone can be positioned in separate pressure chambers and simultaneous positive or negative pressures applied to the bone ends to force fluid through the bone tissue.