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1. US4857163 - High resolution electrophoretic gel and method for separating serum proteins

Office
United States of America
Application Number 07074744
Application Date 17.07.1987
Publication Number 4857163
Publication Date 15.08.1989
Grant Number 4857163
Grant Date 15.08.1989
Publication Kind A
IPC
C07K 14/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
C07K 1/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
C07K 1/26
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
14Extraction; Separation; Purification
24by electrochemical means
26Electrophoresis
G01N 27/447
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
CPC
G01N 27/44747
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44704Details; Accessories
44747Composition of gel or of carrier mixture
Applicants Beckman Instruments, Inc.
Inventors Gurske William A.
Blessum Cynthia R.
Cheng Min-Lee
Andrade Mark S.
Agents May William H.
Grant Arnold
Abers Julia E.
Title
(EN) High resolution electrophoretic gel and method for separating serum proteins
Abstract
(EN)

A novel agarose gel is provided for use in the high resolution electrophoretic (HRE) separation of serum proteins. The agarose gel employs a novel gel buffer which contains either hippurate, glycine, or a mixture thereof, in combination with barbital and Tris. The agarose gel of the present invention yields improved electrophoretic separation of the serum proteins in a sample whether the same gel buffer is also used as the running buffer or whether a standard barbital running buffer is used.