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A system for quantifying the presence of at least one chemical and/or a physical condition in ocular tissues is provided. The system uses a membrane having at least one photo-reactive chemical- and/or physical condition-sensing indicator dye and a photo-reactive reference dye wherein both the photo-reactive chemical- and/or physical condition-sensing indicator dye and the photo-reactive reference dye are responsive to the same wavelength of an excitation light source but emit different wavelengths of light in a light spectrum. An optical data detector receives the emitted light and a processor splits the data into two or more colours of the light spectrum and measures the intensity of the two or more colours. The colour intensity belonging to the emitted light from the chemical- and/or physical condition-sensing indicator dye is compared to the colour intensity of the light emitted from the reference dye and the amount of chemical present and/or the extent of physical condition is ratiometrically determined therefrom.