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1. US20190071682 - 3'-UTR Sequences for Stabilization of RNA

Office
United States of America
Application Number 15763709
Application Date 05.10.2016
Publication Number 20190071682
Publication Date 07.03.2019
Publication Kind A1
IPC
C12N 15/67
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12N 15/113
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113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
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90Stable introduction of foreign DNA into chromosome
C12N 5/0735
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07Animal cells or tissues
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073Embryonic cells or tissues; Foetal cells or tissues
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09Recombinant DNA-technology
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67General methods for enhancing the expression
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09Recombinant DNA-technology
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C12N 2310/113
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2310Structure or type of the nucleic acid
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C12N 5/0606
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5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0603Embryonic cells
0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
Applicants BioNTech RNA Pharmaceuticals GmbH
TRON-Translationale Onkologie an der Universitats- medizin der Johannes Gutenberg-Universitat Mainz
Inventors Alexandra Orlandini Von Niessen
Stephanie Fesser
Britta Vallazza
Tim Beissert
Andreas Kuhn
Ugur Sahin
Marco Alexander Poleganov
Priority Data PCT/EP2015/073180 07.10.2015 EP
Title
(EN) 3'-UTR Sequences for Stabilization of RNA
Abstract
(EN)

The present invention relates to stabilization of RNA, in particular mRNA, and an increase in mRNA translation. The present invention particularly relates to a modification of RNA, in particular in vitro-transcribed RNA, resulting in increased transcript stability and/or translation efficiency. According to the invention, it was demonstrated that certain sequences in the 3′-untranslated region (UTR) of an RNA molecule improve stability and translation efficiency.