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1. (US20180327830) METHOD FOR DETERMINING CELL CLONALITY

Office : United States of America
Application Number: 15776967 Application Date: 01.12.2016
Publication Number: 20180327830 Publication Date: 15.11.2018
Publication Kind : A1
Prior PCT appl.: Application Number:PCTEP2016079427 ; Publication Number: Click to see the data
IPC:
C12Q 1/6874
[IPC code unknown for C12Q 1/6874]
CPC:
C12Q 1/6874
Applicants: ARES TRADING S.A.
Inventors: Fabio LA NEVE
Georg FEGER
Emiliano TOSO
Priority Data: 15197894.7 03.12.2015 EP
Title: (EN) METHOD FOR DETERMINING CELL CLONALITY
Abstract: front page image
(EN)

The invention relates to a method for determining the clonality of a master cell bank (MCB) in which a transgene has been inserted. The method involves a combination of sequencing methodology and bioinformatic analysis, performed on multiple subclones originating from a common MCB, to establish a reliable set of reference transgene insertion regions in one reference subclone, and corresponding sets of comparative transgene insertion regions in one or more other subclones originating from the same MCB. Based on the degree of congruity between reference and comparative transgene insertion regions, the MCB is determined to be either monoclonal or polyclonal.


Also published as:
AU2016363746CA3005386CN108368549KR1020180088867EP3384045IL259750
WO/2017/093400