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1. US20180325120 - Compositions and methods to reduce the population of wheat-stem sawfly and hessian fly

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Claims

1. A biocontrol composition comprising an agriculturally acceptable carrier and an effective amount of at least one endophytic fungal strain that kills wheat stem sawfly larvae and Hessian fly larvae, said at least one endophytic fungal strain is selected from the group consisting of Beauveria pseudobassiana strain CT01 (NRRL Access. No. 67405), Beauveria pseudobassiana strain CT10 (NRRL Access. No. 67401), Beauveria amorpha strain CT14 (NRRL Access. No. 67402), Beauveria bassiana strain 15CT13 (NRRL Access. No. 67609), Beauveria bassiana strain 15CT44 (NRRL Access. No. 67610), Beauveria bassiana strain 16WS07 (NRRL Access. No. 67611), Metarhizium pemphigi strain CT19 (NRRL Access. No. 67403), Metarhizium pemphigi strain WS64 (NRRL Access. No. 67404), and Metarhizium anisopliae strain 16WS10 (NRRL Access. No. 67612).
2. The biocontrol composition of claim 1, wherein said effective amount of said endophytic fungal strain is between 10 4 to 10 12 spores per dose unit.
3. The biocontrol composition of claim 1, wherein said effective amount of said endophytic fungal strain is between 10 4 to 10 11 CFU vegetative mycelium of said fungal strain per dose unit.
4. The biocontrol composition of claim 1, wherein said agriculturally acceptable carrier is selected from the group consisting of a polymer, a vegetable oil, a vegetable wax, a paraffinic oil, a paraffinic wax, an emulsion of oil and water, an aqueous solution containing a polymer, agar, gelatin, a lipophilic substance that assists the fungi in adhering to the plant or part thereof, and a plant seed.
5. The biocontrol composition of claim 4, wherein said plant seed is a grain crop seed.
6. A kit comprising a first container comprising at least one endophytic fungus that kills wheat stem sawfly larvae or Hessian fly larvae, optionally a second container, and instructions of applying said at least one endophytic fungus to land or a plant; wherein said at least one endophytic fungus is selected from the group consisting of Beauveria pseudobassiana strain CT01 (NRRL Access. No. 67405), Beauveria pseudobassiana strain CT10 (NRRL Access. No. 67401), Beauveria amorpha strain CT14 (NRRL Access. No. 67402), Beauveria bassiana strain 15CT13 (NRRL Access. No. 67609), Beauveria bassiana strain 15CT44 (NRRL Access. No. 67610), Beauveria bassiana strain 16WS07 (NRRL Access. No. 67611), Metarhizium pemphigi strain CT19 (NRRL Access. No. 67403), Metarhizium pemphigi strain WS64 (NRRL Access. No. 67404), and Metarhizium anisopliae strain 16WS10 (NRRL Access. No. 67612).
7. The kit of claim 6, wherein said first container comprises spores or vegetative mycelium of said endophytic fungus.
8. The kit of claim 6, wherein said optional second container comprises an agriculturally acceptable carrier.
9. The kit of claim 8, wherein said agriculturally acceptable carrier is selected from the group consisting of a polymer, a vegetable oil, a vegetable wax, a paraffinic oil, a paraffinic wax, an emulsion of oil and water, an aqueous solution containing a polymer, agar, gelatin, and a lipophilic substance that assists the fungi in adhering to the plant or part thereof.