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1. US20180080052 - Host cells and methods for producing isopentenol from mevalonate

Office United States of America
Application Number 15682325
Application Date 21.08.2017
Publication Number 20180080052
Publication Date 22.03.2018
Grant Number 10273506
Grant Date 30.04.2019
Publication Kind B2
IPC
C CHEMISTRY; METALLURGY
12
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
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FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
02
containing a hydroxy group
04
acyclic
C CHEMISTRY; METALLURGY
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ORGANIC CHEMISTRY
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ACYCLIC, CARBOCYCLIC, OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
9
Compounds containing elements of the 5th Group of the Periodic System
02
Phosphorus compounds
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without P-C bonds
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Esters of oxyacids of phosphorus
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Esters of phosphoric acids
C CHEMISTRY; METALLURGY
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ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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136
Homopolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds
02
the radical having only two carbon-to-carbon double bonds
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conjugated
08
Isoprene
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5
Preparation of hydrocarbons
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Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
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acting on ester bonds (3.1)
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Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
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acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
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9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10
Transferases (2.)
12
transferring phosphorus containing groups, e.g. kinases (2.7)
C12N 1/20
C12P 7/04
C07F 9/09
C08F 136/08
C12P 5/00
C12N 9/16
CPC
C07F 9/091
C07F 9/098
C08F 136/08
C12N 9/0006
C12N 9/1025
C12N 9/1205
Applicants The Regents of the University of California
Inventors Taek Soon Lee
Aram Kang
Agents Robin C. Chang
Lawrence Berkeley National Laboratory
Title
(EN) Host cells and methods for producing isopentenol from mevalonate
Abstract
(EN)

The present invention provides for a genetically modified host cell capable of producing isopentenol and/or 3-methyl-3-butenol, comprising (a) an increased expression of phosphomevalonate decarboxylase (PMD) (b) an increased expression of a phosphatase capable of converting isopentenol into 3-methyl-3-butenol, (c) optionally the genetically modified host cell does not express, or has a decreased expression of one or more of NudB, phosphomevalonate kinase (PMK), and/or PMD, and (d) optionally one or more further enzymes capable of converting isopentenol and/or 3-methyl-3-butenol into a third compound, such as isoprene.