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1. (US20160060637) Improved Gene Targeting and Nucleic Acid Carrier Molecule, In Particular for Use in Plants

Office : United States of America
Application Number: 14786624 Application Date: 23.04.2014
Publication Number: 20160060637 Publication Date: 03.03.2016
Publication Kind : A1
Prior PCT appl.: Application Number:PCTEP2014058246 ; Publication Number: Click to see the data
IPC:
C12N 15/64
C07K 14/47
C12N 9/16
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
64
General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
46
from vertebrates
47
from mammals
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
CPC:
C12N 15/64
C12N 9/16
C07K 14/47
C07K 231/02
Applicants: RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITÄT BONN
Rheinische Friedrich-Wilhelms-Universitaet Bonn
Inventors: Carl HOMMELSHEIM
Lamprinos FRANTZESKAKIS
Bekir UELKER
Priority Data: 13164966.7 23.04.2013 EP
Title: (EN) Improved Gene Targeting and Nucleic Acid Carrier Molecule, In Particular for Use in Plants
Abstract: front page image
(EN)

The present invention relates to a nucleic acid carrier molecule, comprising the general formula M-S1-L-W—S2, wherein M is a first polypeptide specifically binding to a donor nucleic acid sequence to be transferred into an organelle of a cell, W is a second polypeptide specifically binding to a target nucleic acid sequence, wherein said target nucleic acid sequence is located in an organelle of a cell, L is missing or is linking group allowing M and W flexibility and semi-independence, and S1 and S2 independently of each other are missing or are a signal peptide sequence, and can be fused to M and W proteins either N- or C-terminally, wherein said donor nucleic acid sequence is brought into close proximity with said target nucleic acid sequence when both nucleic acid sequences are bound to said carrier molecule. The present invention furthermore relates to methods for recombinantly transforming a nucleic acid into an organelle in a cell, preferably a plant cell, employing said nucleic acid carrier molecule.


Also published as:
EP2989206WO/2014/173955