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1. US20150247118 - METHOD FOR PRODUCING 3D CELL CULTURE

Office
United States of America
Application Number 14427536
Application Date 12.09.2013
Publication Number 20150247118
Publication Date 03.09.2015
Publication Kind A1
IPC
C12N 5/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
CPC
C12N 5/0062
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0062General methods for three-dimensional culture
Applicants OSAKA UNIVERSITY
MITSUBISHI PAPER MILLS LIMITED
Inventors Mitsuru Akashi
Michiya Matsusaki
Atsushi Matsuzawa
Priority Data 2012203154 14.09.2012 JP
Title
(EN) METHOD FOR PRODUCING 3D CELL CULTURE
Abstract
(EN)

Provided is a novel method for producing a three-dimensional cell culture construct. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; culturing the seeded coated cells in a culture medium; and using at least a portion of the culture medium continuously for 5 days or more. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; and culturing the seeded coated cells in a culture medium. The culture process of the coated cells includes culturing the coated cells in 70 μL or more of the culture medium per 1×104 cells.


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