US20140363875 NOVEL DNA POLYMERASE

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United States of America

Application Number 14130636

Application Date 12.07.2012

Publication Number 20140363875

Publication Date 11.12.2014

Publication Kind A1

IPC

CPC

Applicants Ishino Yoshizumi
KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION
Yamagami Takeshi
Matsukawa Hiroaki

Inventors Ishino Yoshizumi
Yamagami Takeshi
Matsukawa Hiroaki

Priority Data 2011-153410 12.07.2011 JP

Title

(EN) NOVEL DNA POLYMERASE

Abstract

(EN)

Provided are various novel DNA polymerases.

Provided are: a DNA polymerase comprising: an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10	Transferases (2.)
12	transferring phosphorus containing groups, e.g. kinases (2.7)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10	Transferases (2.)
12	transferring phosphorus containing groups, e.g. kinases (2.7)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes; Proenzymes; Compositions thereof
10	Transferases (2.)
12	transferring phosphorus containing groups, e.g. kinases (2.7)
1241	Nucleotidyltransferases (2.7.7)
1252	DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes; Proenzymes; Compositions thereof
10	Transferases (2.)
12	transferring phosphorus containing groups, e.g. kinases (2.7)
1241	Nucleotidyltransferases (2.7.7)
1252	DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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1. US20140363875 - NOVEL DNA POLYMERASE