US20140322793 DNA polymerases

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United States of America

Application Number 14232174

Application Date 12.07.2012

Publication Number 20140322793

Publication Date 30.10.2014

Grant Number 09447388

Grant Date 20.09.2016

Publication Kind B2

IPC

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Applicants Yoshizumi Ishino
KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION
Takeshi Yamagami
TAKARA BIO INC.
Hiroaki Matsukawa
Takashi Uemori
Takehiro Sagara

Inventors Yoshizumi Ishino
Takeshi Yamagami
Hiroaki Matsukawa
Takashi Uemori
Takehiro Sagara

Agents Birch, Stewart, Kolasch & Birch, LLP

Priority Data 2011-153410 12.07.2011 JP

Title

(EN) DNA polymerases

Abstract

(EN)

Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A₇₃₇-A₇₃₈-A₇₃₉-A₇₄₀-A₇₄₁-A₇₄₂-A₇₄₃-A₇₄₄-A₇₄₅-” between the amino acid residue at position 736 and the amino acid residue at position 737,

wherein:

- A₇₃₇is an amino acid residue having a non-polar aliphatic side chain;
- A₇₃₈is an amino acid residue having a non-polar aliphatic side chain;
- A₇₃₉is an amino acid residue having a positively charged side chain;
- A₇₄₀is an amino acid residue having a positively charged side chain;
- A₇₄₁is an amino acid residue having a non-polar aliphatic side chain;
- A₇₄₂is an amino acid residue having a non-polar aliphatic side chain;
- A₇₄₃is any given amino acid residue;
- A₇₄₄is an amino acid residue having a positively charged side chain; and
- A₇₄₅is an amino acid residue having a non-polar aliphatic side chain).

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10	Transferases (2.)
12	transferring phosphorus containing groups, e.g. kinases (2.7)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1	Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1	Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20	Bacteria; Culture media therefor

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19	Preparation of compounds containing saccharide radicals
26	Preparation of nitrogen-containing carbohydrates
28	N-glycosides
30	Nucleotides
34	Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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1. US20140322793 - DNA polymerases

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Y	ENZYMES
207	Transferases transferring phosphorus-containing groups (2.7)
07	Nucleotidyltransferases (2.7.7)
07007	DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Y	SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
02	TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
P	CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
20	Technologies relating to chemical industry
50	Improvements relating to the production of bulk chemicals
52	using catalysts, e.g. selective catalysts