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1. US20140189895 - TECHNIQUE FOR CLEAVING OUT PART OF POLY(A) CHAIN AND/OR 3'-TERMINAL SEQUENCE OF mRNA TO INHIBIT TRANSLATION REACTION

Office
United States of America
Application Number 14234060
Application Date 17.07.2012
Publication Number 20140189895
Publication Date 03.07.2014
Publication Kind A1
IPC
C12N 15/11
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
CPC
C12N 15/111
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
111General methods applicable to biologically active non-coding nucleic acids
Applicants Wada Tadashi
YOSHINDO INC.
Takeda Kei
PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY
Handa Hiroshi
TOKYO INSTITUTE OF TECHNOLOGY
Inventors Wada Tadashi
Takeda Kei
Handa Hiroshi
Priority Data 2011-160512 22.07.2011 JP
Title
(EN) TECHNIQUE FOR CLEAVING OUT PART OF POLY(A) CHAIN AND/OR 3'-TERMINAL SEQUENCE OF mRNA TO INHIBIT TRANSLATION REACTION
Abstract
(EN)

The present invention provides a method of artificially repressing gene expression, which is simpler to design than conventional methods (the RNAi, ribozyme and antisense methods) and which allows for easier confirmation of the effect.

A method of inhibiting the translation reaction of a target gene, comprising cutting out a part of the poly(A) tail and/or 3′-terminal sequence of the target mRNA is provided. Also provided are a kit for inhibiting the translation reaction of a target gene, comprising a reagent capable of cutting out a part of the poly(A) tail and/or 3′-terminal sequence of the target mRNA; a cell in which the translation reaction of a target gene is inhibited by introduction thereinto of a reagent capable of cutting out a part of the poly(A) tail and/or 3′-terminal sequence of the target mRNA; and a non-human organism in which the translation reaction of a target gene is inhibited by introduction thereinto of a reagent capable of cutting out a part of the poly(A) tail and/or 3′-terminal sequence of the target mRNA.