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1. RU0002758932 - METHOD FOR MEASURING MASS CONCENTRATION OF METHYL ESTERS OF FATTY ACIDS IN BIOLOGICAL MEDIA BY GAS-LIQUID CHROMATOGRAPHY METHOD

Office
Russian Federation
Application Number 2020124879
Application Date 17.07.2020
Publication Number 0002758932
Publication Date 03.11.2021
Grant Number
Grant Date 03.11.2021
Publication Kind C1
IPC
G01N 33/12
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
02Food
12Meat; Fish
G01N 33/483
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
G01N 33/487
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
G01N 33/49
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
49blood
G01N 30/02
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
CPC
G01N 30/02
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography ; or field flow fractionation;
02Column chromatography
G01N 33/12
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
02Food
12Meat; fish
G01N 33/483
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
483Physical analysis of biological material
G01N 33/487
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
483Physical analysis of biological material
487of liquid biological material
G01N 33/49
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
483Physical analysis of biological material
487of liquid biological material
49Blood
Inventors Бичкаева Фатима Артёмовна (RU)
Баранова Нина Федотовна (RU)
Власова Ольга Сергеевна (RU)
Нестерова Екатерина В. (RU)
Бичкаев Артём Альбертович (RU)
Шенгоф Борис Александрович (RU)
Третьякова Татьяна Васильевна (RU)
Bichkaeva Fatima Artemovna (RU)
Baranova Nina Fedotovna (RU)
Vlasova Olga Sergeevna (RU)
Nesterova Ekaterina V. (RU)
Bichkaev Artem Albertovich (RU)
Shengof Boris Aleksandrovich (RU)
Tretyakova Tatyana Vasilevna (RU)
Title
(EN) METHOD FOR MEASURING MASS CONCENTRATION OF METHYL ESTERS OF FATTY ACIDS IN BIOLOGICAL MEDIA BY GAS-LIQUID CHROMATOGRAPHY METHOD
(RU) Способ измерения массовой концентрации метиловых эфиров жирных кислот в биологических средах методом газожидкостной хроматографии
Abstract
(EN) FIELD: chemistry. SUBSTANCE: invention relates to various fields of the national economy (medicine, chemical and pharmaceutical industries), wherein there is a demand for measuring the mass concentration of methyl esters of fatty acids (MEFA) in biological media, including animal and plant tissues, by the gas-liquid chromatography method. The method for measuring the mass concentration of methyl esters of fatty acids (MEFA) in biological fluids by the gas-liquid chromatography method includes extraction of lipids, methylation of fatty acids by means of interesterification of lipids in the presence of an acidic catalyst, extraction of MEFA, reduction of the extracts of lipids and MEFA by variants at atmospheric pressure or under a vacuum, identification and measurement of the mass concentration of methyl esters of 43 fatty acids in the range of 0.3 to 1,000 mcg/cm3 using gas-liquid chromatography by the internal standard method, wherein fluid media of saliva, hemolymph, tissues of plants, fish or animals are used as the biological media; homogenisation of tissue samples and weighing thereof with an accuracy of up to 0.0002 g is conducted in the process of sample preparation, followed by calculatin ` `4saqg the mass concentration of each MEFA in mcg/g by the formula: wherein: X is the mass concentration of each MEFA in the sample, mcg/g; A is the ratio of the detector response areas for this MEFA to the standard; B is the calibration coefficient for this MEFA; C is the amount of the internal standard added 4to the sample weight, mcg; K is the coefficient of reduction of the measurement result to 1 g (K=1/analysis sample weight, g). The result is achieved by applying vacuum equipment, thus increasing the quantitative yield of MEFA by 1.2 ÷1.7 times, thereby increasing the sensitivity and accuracy of the method. EFFECT: invention provides a possibility of isolating and quantifying the MEFA even in small doses of biological samples, with a low content of fatty acids in the sample. 1 cl, 6 tbl, 6 ex
(RU) Изобретение относится к различным областям народного хозяйства (медицине, химической и фармацевтической промышленности), где есть потребность в измерении массовой концентрации метиловых эфиров жирных кислот (МЭЖК) в биологических средах, в том числе животных и растительных тканях методом газожидкостной хроматографии. Способ измерений массовой концентрации метиловых эфиров жирных кислот (МЭЖК) в биологических средах методом газожидкостной хроматографии включает экстракцию липидов, метилирование жирных кислот путем переэтерификации липидов в присутствии кислотного катализатора, экстракцию МЭЖК, упаривание экстрактов липидов и МЭЖК вариантами под атмосферным давлением или под вакуумом, идентификацию и измерение массовой концентрации метиловых эфиров 43 жирных кислот в диапазоне 0,3-1000 мкг/см3 с помощью газожидкостной хроматографии методом внутреннего стандарта, где в качестве биологических сред используются жидкие среды слюны, гемолимфы, ткани растений, рыб или животных; в процессе подготовки проб выполняется гомогенизация образцов тканей и их взвешивание с точностью до 0,0002 г, с последующим расчетом массовой концентрации каждого МЭЖК в мкг/г по формуле: где: Х - массовая концентрация каждого МЭЖК в пробе, мкг/г; А - отношение площадей откликов детектора для данного МЭЖК к стандарту; В - калибровочный коэффициент для данного МЭЖК; С - количество внутреннего стандарта, добавленного к навеске пробы, мкг; К - коэффициент приведения результата измерения к 1 г (К=1 / масса навески на анализ, г). Изобретение позволяет выделить и количественно измерить МЭЖК даже в малых дозах биологических образцов, низком содержании жирных кислот в образце. Это достигается путем применения вакуумного оборудования, что увеличивает количественный выход МЭЖК в 1,2÷1,7 раза, повышая тем самым чувствительность и достоверность способа. 6 табл., 6 пр.