RU02385348 EXPRESSING SYSTEM

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Russian Federation

Application Number 2004134341/13

Application Date 06.07.2001

Publication Number 02385348

Publication Date 27.03.2010

Grant Number

Grant Date 27.03.2010

Publication Kind C2

IPC

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CPC

Inventors УИЛЛЬЯМСОН Этел Дайан (GB)
МИЛЛЕР Джули (GB)
УОЛКЕР Никола Джейн (GB)
БЭЙЛЛИ Лесли Вилльям Джеймс (GB)
ХОЛДЕН Паула Томсон (GB)
ФЛИК-СМИТ Хелен Клэйр (GB)
БАЛЛИФЕНТ Хелен Лайза (GB)
ТИТБОЛЛ Ричард Вилльям (GB)
ТОППИНГ Эндрю Вилльям (GB)
UILL'JaMSON Ehtel Dajan (GB)
MILLER Dzhuli (GB)
UOLKER Nikola Dzhejn (GB)
BEhJLLI Lesli Vill'jam Dzhejms (GB)
KhOLDEN Paula Tomson (GB)
FLIK-SMIT Khelen Klehjr (GB)
BALLIFENT Khelen Lajza (GB)
TITBOLL Richard Vill'jam (GB)
TOPPING Ehndrju Vill'jam (GB)

Priority Data 0016702.3 08.07.2000 GB

Title

(EN) EXPRESSING SYSTEM

(RU) ЭКСПРЕССИРУЮЩАЯ СИСТЕМА

Abstract

(EN) FIELD: medicine. SUBSTANCE: invention concerns the method for making an immunogenic reagent which causes immune response on infection Bacillus anthracis, including one to several polypeptides which together represent three domains of a full-size protective antigen (PA) from B anthracis or their versions, and at least, one of specified domains contains domain 1 or domain 4 of the PA, or its version. Said polypeptides of specified immunogenic reagent, and the full-size PA are produced as a result of expression in a recombinant cell E.coli. The invention also discloses an expression vector and nucleic acid with percent of residual guanidine and cytosine more than 35%, coding immunigenic polypeptide which is said protective antigen (PA). EFFECT: high-yield immunogenic polypeptide. 13 cl, 5 dwg, 3 tbl, 6 ex

(RU) Изобретение касается способа получения иммуногенного реагента, который вызывает иммунный ответ на инфекцию Bacillus anthracis, включающего в себя один или несколько полипептидов, которые вместе представляют три домена полноразмерного защитного антигена (РА) из В. anthracis или их варианты, и по меньшей мере один из указанных доменов включает в себя домен 1 или домен 4 РА или его вариант. Данные полипептиды указанного иммуногенного реагента, а также полноразмерный РА продуцируются в результате экспрессии в рекомбинантной клетке E.coli. Изобретение раскрывает также вектор экспрессии и нуклеиновую кислоту с процентной долей остатков гуанидина и цитозина более 35%, кодирующую иммуногенный полипептид, являющийся защитным антигеном (РА). Использование данного способа обеспечивает высокий выход иммуногенного полипептида. 4 н. и 9 з.п. ф-лы, 5 ил., 4 табл.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70	Vectors or expression systems specially adapted for E. coli

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
11	DNA or RNA fragments; Modified forms thereof
31	Genes encoding microbial proteins, e.g. enterotoxins

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1	Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20	Bacteria; Culture media therefor
21	modified by introduction of foreign genetic material

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21	Preparation of peptides or proteins

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70	Vectors or expression systems specially adapted for E. coli

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1. RU02385348 - EXPRESSING SYSTEM

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
K	PEPTIDES
14	Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195	from bacteria
32	from Bacillus (G)

A	HUMAN NECESSITIES
61	MEDICAL OR VETERINARY SCIENCE; HYGIENE
K	PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39	Medicinal preparations containing antigens or antibodies