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1. PT106959 - PROCESSOS PARA A PRODUÇÃO DE GLICOLÍPIDOS MICROBIANOS DO TIPO MANOSILERITRITOLÍPIDOS, A PARTIR DE MATERIAIS LENHOCELULÓSICOS E SUAS APLICAÇÕES

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Office
Portugal
Application Number 106959
Application Date 17.05.2013
Publication Number 106959
Publication Date 17.11.2014
Grant Number 106959
Grant Date 20.04.2020
Publication Kind B
IPC
C12P 19/44
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
44Preparation of O-glycosides, e.g. glucosides
CPC
C12P 7/62
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
62Carboxylic acid esters
C12P 19/44
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
44Preparation of O-glycosides, e.g. glucosides
C12P 2203/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
2203Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
C12P 7/64
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
C12P 2201/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
2201Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
Applicants INSTITUTO SUPERIOR TÉCNICO
LABORATÓRIO NACIONAL DE ENERGIA E GEOLOGIA
Inventors CÉSAR SIMÕES DA FONSECA
NUNO RICARDO FARIA
FREDERICO CASTELO ALVES FERREIRA
Title
(PT) PROCESSOS PARA A PRODUÇÃO DE GLICOLÍPIDOS MICROBIANOS DO TIPO MANOSILERITRITOLÍPIDOS, A PARTIR DE MATERIAIS LENHOCELULÓSICOS E SUAS APLICAÇÕES
Abstract
(EN) The present invention relates to processes for the production of microbial glycolipids, mannosylerythritol lipids (MEL), from lignocellulosic carbon source. These processes are characterized in that the use of lignocellulosic materials for the production of a microbial glycolipids, MEL, comprising a fermentation preferably using fungi of the genus Pseudozyma or other microorganisms such as genetically modified fungi or bacteria. The processes for production of microbial glycolipids, MEL comprise three steps: pretreatment of lignocellulosic material; enzymatic hydrolysis; and. fermentation. The enzymatic hydrolysis and fermentation may take place sequentially or simultaneously with addition of exogenous enzymes or simultaneously with enzymes produced by the microorganism itself. The produced microbial glycolipids have applications as: biosurfactants; antimicrobials; anticancer agents; wound healing factors; stabilizer agents on storage and purification of proteins or vaccines; drugs and gene deliver agents; antifreeze agents.
(PT) A PRESENTE INVENÇÃO REFERE-SE A PROCESSOS PARA A PRODUÇÃO DE GLICOLÍPIDOS MICROBIANOS, DO TIPO MANOSILERITRITOLÍPIDOS (MEL), A PARTIR DE FONTES DE CARBONO DE ORIGEM LENHOCELULÓSICA. ESTES PROCESSOS SÃO CARACTERIZADOS POR UTILIZAR MATERIAIS LENHOCELULÓSICOS E POR ESTAREM ENVOLVIDOS NA FERMENTAÇÃO PREFERENCIALMENTE FUNGOS DO GÉNERO PSEUDOZYMA OU OUTROS MICRORGANISMOS COMO FUNGOS OU BACTÉRIAS GENETICAMENTE MODIFICADOS E POR SE PRODUZIREM GLICOLÍPIDOS MICROBIANOS DO TIPO MEL. OS PROCESSOS DE PRODUÇÃO DE GLICOLÍPIDOS MICROBIANOS DO TIPO MEL INCLUEM TRÊS ETAPAS: PRÉ-TRATAMENTO DO MATERIAL LENHOCELULÓSICO; HIDRÓLISE ENZIMÁTICA; E FERMENTAÇÃO. A HIDRÓLISE ENZIMÁTICA E A FERMENTAÇÃO PODEM DECORRER DE FORMA SEQUENCIAL OU EM SIMULTÂNEO COM O AUXÍLIO DE ENZIMAS EXÓGENAS, OU EM SIMULTÂNEO COM ENZIMAS PRODUZIDAS PELO PRÓPRIO MICRORGANISMO. OS GLICOLÍPIDOS MICROBIANOS PRODUZIDOS TÊM APLICAÇÃO PARA PRODUTOS: BIOSURFACTANTES; ANTIMICROBIANOS; ANTICANCERÍGENOS; PARA REGENERAÇÃO EPITELIAL; PARA ESTABILIZAÇÃO E/OU PURIFICAÇÃO DE PROTEÍNAS OU VACINAS; PARA ADMINISTRAÇÃO DE FÁRMACOS E GENES; ANTICONGELANTES.
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