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1. WO2017036495 - METHOD OF FERMENTATIVE ALPHA-IONONE PRODUCTION

Publication Number WO/2017/036495
Publication Date 09.03.2017
International Application No. PCT/EP2015/069751
International Filing Date 28.08.2015
IPC
C12P 23/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
23Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
C12N 15/63 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12P 5/00 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
5Preparation of hydrocarbons
CPC
C12N 1/205
C12N 15/63
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/74
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N 9/0069
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0069acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
C12N 9/10
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9Enzymes; Proenzymes; Compositions thereof
90Isomerases (5.)
Applicants
  • PHYTOWELT GREENTECHNOLOGIES GMBH [DE]/[DE]
Inventors
  • JACH, Guido
  • AZDOUFFAL, Sanae
  • SCHULLEHNER, Katrin
  • WELTERS, Peter
  • NATANEK, Angela
Agents
  • ZIMMER, Franz-Josef
Priority Data
Publication Language German (de)
Filing Language German (DE)
Designated States
Title
(DE) VERFAHREN ZUR FERMENTATIVEN ALPHA-IONEN PRODUKTION
(EN) METHOD OF FERMENTATIVE ALPHA-IONONE PRODUCTION
(FR) PROCÉDÉ DE PRODUCTION PAR FERMENTATION D'ALPHA-IONS
Abstract
(DE) Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung von enantiomerenreinem alphaIonon. Des Weiteren betrifft die Erfindung eine Nukleinsäure, welche eine Sequenz umfasst, die eine Lycopen-epsilon-Zyklase (EC) kodiert, eine Lycopen-epsilon-Zyklase (EC), Plasmide, die Komponenten der alpha-Ionon Biosynthese kodieren und einen Mikroorganismus, welcher heterologe Nukleotidsequenzen enthält, die die Enzyme Geranylgeranyl-Diphosphat-Synthase, Isopentenyl-Diphosphat-Isomerase (IPI), Phytoen Desaturase/Dehydrogenase (crtl), Phytoensynthase (crt B) und Lycopen-epsilon-Zyklase (EC) oder Geranylgeranyl-Diphosphat- Synthase, Isopentenyl-Diphosphat-Isomerase (IPI), Phytoen Desaturase/Dehydrogenase (crtl), Phytoensynthase (crt B), Lycopen-epsilon-Zyklase (EC) und Carotenoid-Cleavage-Dioxygenase (CCD1) kodieren. Ferner betrifft die Erfindung ein Verfahren zur Herstellung von hochreinem epsilon-Carotin.
(EN) The present invention relates to a method of preparing enantiomerically pure alpha-ionone. The invention further relates to a nucleic acid comprising a sequence that encodes a lycopene epsilon-cyclase (EC), a lycopene epsilon-cyclase (EC), plasmids that encode components of alpha-ionone biosynthesis and a microorganism containing heterologous nucleotide sequences that encode the enzymes geranylgeranyl diphosphate synthase, isopentenyl diphosphate isomerase (IPI), phytoene desaturase/dehydrogenase (crtl), phytoene synthase (crt B) and lycopene epsilon-cyclase (EC) or geranylgeranyl diphosphate synthase, isopentenyl diphosphate isomerase (IPI), phytoene desaturase/dehydrogenase (crtl), phytoene synthase (crt B), lycopene epsilon-cyclase (EC) and carotenoid cleavage dioxygenase (CCD1). The invention further relates to a method of producing high-purity epsilon-carotene.
(FR) La présente invention concerne un procédé de production d'alpha-ions énantiopurs. L'invention concerne également un acide nucléique comprenant une séquence codant pour la lycopène epsilon cyclase (EC), la lycopène epsilon cyclase (EC), des plasmides codant pour les composants de la biosynthèse d'alpha-ions et un microorganisme qui contient des séquences nucléotidiques hétérologues codant pour les enzymes géranylgéranyl diphosphate synthase, isopentényl diphosphate isomérase (IPI), phytoène désaturase/déshydrogénase (crtl), phytoène synthase (crt B) et lycopène epsilon cyclase (EC) ou géranylgéranyl diphosphate synthase, isopentényl diphosphate isomérase (IPI), phytoène désaturase/déshydrogénase (crtl), la phytoène synthase (crt B), la lycopène epsilon cyclase (EC) et la caroténoïde cléavage dioxygénase (CCD1). L'invention concerne également un procédé de production d'epsilone carotène de grande pureté.
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